TY - JOUR
T1 - RN-tre identifies a family of tre-related proteins displaying a novel potential protein binding domain
AU - Matòšková, Broňa
AU - Wong, William T.
AU - Seki, Naohiko
AU - Nagase, Takahiro
AU - Nomura, Nobuo
AU - Robbins, Keith C.
AU - Di Fiore, Pier Paolo
PY - 1996
Y1 - 1996
N2 - Eps8 is a recently identified SH3-containing substrate for tyrosine kinase receptors. To understand the role of eps8 in receptor-mediated signaling, we cloned cDNAs encoding proteins that bind to its SH3 domain. One of these cDNAs predicts the synthesis of an 828 amino acid protein with homology to the N-terminal region of the tre oncogene. We designated this protein RN-tre for Related to the N-terminus of tre. RN-tre is ubiquitously expressed and maps to 10p13, a region known to be involved in translocations in various leukemias. In addition, a 10p13 monosomy syndrome, characterized by developmental alterations, has been reported. The regional homology between RN-tre and tre, which is limited to their N-terminal portion, prompted us to investigate the origin of the tre oncogene transcriptional unit. We were able to show that tre is the fusion product of a 5' genetic element, homologous to RN-tre and a 3' element, encoding a de-ubiquinating enzyme. Moreover, we identified, within the N-terminus of RN-tre and tre, a domain (named TrH, for Tre Homology), which is conserved within several proteins from yeast to mammals and has protein-binding properties in vitro.
AB - Eps8 is a recently identified SH3-containing substrate for tyrosine kinase receptors. To understand the role of eps8 in receptor-mediated signaling, we cloned cDNAs encoding proteins that bind to its SH3 domain. One of these cDNAs predicts the synthesis of an 828 amino acid protein with homology to the N-terminal region of the tre oncogene. We designated this protein RN-tre for Related to the N-terminus of tre. RN-tre is ubiquitously expressed and maps to 10p13, a region known to be involved in translocations in various leukemias. In addition, a 10p13 monosomy syndrome, characterized by developmental alterations, has been reported. The regional homology between RN-tre and tre, which is limited to their N-terminal portion, prompted us to investigate the origin of the tre oncogene transcriptional unit. We were able to show that tre is the fusion product of a 5' genetic element, homologous to RN-tre and a 3' element, encoding a de-ubiquinating enzyme. Moreover, we identified, within the N-terminus of RN-tre and tre, a domain (named TrH, for Tre Homology), which is conserved within several proteins from yeast to mammals and has protein-binding properties in vitro.
KW - eps8
KW - SH3
KW - Signal transduction
KW - tre
UR - http://www.scopus.com/inward/record.url?scp=0029889257&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029889257&partnerID=8YFLogxK
M3 - Article
C2 - 8700515
AN - SCOPUS:0029889257
VL - 12
SP - 2563
EP - 2571
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 12
ER -