RNA interference for the identification of disease-associated genes

Alessio Nencioni, Peter Sandy, Christopher Dillon, Stephan Kissler, Peter Blume-Jensen, Luk Van Parijs

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

RNA interference (RNAi) has emerged as a novel cellular mechanism regulating gene expression at the post-transcriptional level and as a powerful tool to control gene function experimentally. Recent advances in the biology and application of RNAi include the definition of improved criteria for selecting effective small interfering RNA (siRNA) sequences, and the generation of vectors for the delivery of siRNAs and stable silencing of genes in mammalian cells, tissues and animals. High-throughput screening projects based on RNAi have been initiated to search for genes involved in basic biological processes and in complex pathological conditions such as cancer, autoimmunity and degenerative disorders. This research is helping to identify novel therapeutic targets for a range of diseases and may translate into novel clinical applications for RNAi.

Original languageEnglish
Pages (from-to)136-140
Number of pages5
JournalCurrent Opinion in Molecular Therapeutics
Volume6
Issue number2
Publication statusPublished - Apr 2004

Fingerprint

RNA Interference
Genes
Biological Phenomena
Gene Silencing
Autoimmunity
Small Interfering RNA
Gene Expression
Research
Neoplasms
Therapeutics

Keywords

  • Disease-associated genes
  • Genetic screens
  • Retroviral vectors
  • RNA interference
  • Short hairpin RNAs
  • Small interfering RNAs

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Molecular Medicine
  • Genetics(clinical)
  • Drug Discovery
  • Pharmacology
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Nencioni, A., Sandy, P., Dillon, C., Kissler, S., Blume-Jensen, P., & Van Parijs, L. (2004). RNA interference for the identification of disease-associated genes. Current Opinion in Molecular Therapeutics, 6(2), 136-140.

RNA interference for the identification of disease-associated genes. / Nencioni, Alessio; Sandy, Peter; Dillon, Christopher; Kissler, Stephan; Blume-Jensen, Peter; Van Parijs, Luk.

In: Current Opinion in Molecular Therapeutics, Vol. 6, No. 2, 04.2004, p. 136-140.

Research output: Contribution to journalArticle

Nencioni, A, Sandy, P, Dillon, C, Kissler, S, Blume-Jensen, P & Van Parijs, L 2004, 'RNA interference for the identification of disease-associated genes', Current Opinion in Molecular Therapeutics, vol. 6, no. 2, pp. 136-140.
Nencioni A, Sandy P, Dillon C, Kissler S, Blume-Jensen P, Van Parijs L. RNA interference for the identification of disease-associated genes. Current Opinion in Molecular Therapeutics. 2004 Apr;6(2):136-140.
Nencioni, Alessio ; Sandy, Peter ; Dillon, Christopher ; Kissler, Stephan ; Blume-Jensen, Peter ; Van Parijs, Luk. / RNA interference for the identification of disease-associated genes. In: Current Opinion in Molecular Therapeutics. 2004 ; Vol. 6, No. 2. pp. 136-140.
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