RNA sequencing identifies specific PIWI-interacting small noncoding RNA expression patterns in breast cancer

Adnan Hashim, Francesca Rizzo, Giovanna Marchese, Maria Ravo, Roberta Tarallo, Giovanni Nassa, Giorgio Giurato, Gianluca Santamaria, Angela Cordella, Concita Cantarella, Alessandro Weisz

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

PIWI-interacting small non-coding RNAs (piRNAs) are genetic and epigenetic regulatory factors in germline cells, where they maintain genome stability, are involved in RNA silencing and regulate gene expression. We found that the piRNA biogenesis and effector pathway are present in human breast cancer (BC) cells and, analyzing smallRNA-Seq data generated from BC cell lines and tumor biopsies, we identified >100 BC piRNAs, including some very abundant and/or differentially expressed in mammary epithelial compared to BC cells, where this was influenced by estrogen or estrogen receptor β, and in cancer respect to normal breast tissues. A search for mRNAs targeted by the BC piRNome revealed that eight piRNAs showing a specific expression pattern in breast tumors target key cancer cell pathways. Evidence of an active piRNA pathway in BC suggests that these small non-coding RNAs do exert transcriptional and post-transcriptional gene regulatory actions also in cancer cells.

Original languageEnglish
Pages (from-to)9901-9910
Number of pages10
JournalOncotarget
Volume5
Issue number20
Publication statusPublished - 2014

Fingerprint

RNA Sequence Analysis
Small Untranslated RNA
Breast Neoplasms
Small Interfering RNA
Breast
Neoplasms
Genomic Instability
Regulator Genes
RNA Interference
Tumor Cell Line
Epigenomics
Estrogen Receptors
Estrogens
Biopsy
Gene Expression
Messenger RNA

Keywords

  • Breast cancer
  • Estrogen receptor
  • piRNAs
  • PIWI
  • RNA-sequencing
  • Small non-coding RNA

ASJC Scopus subject areas

  • Oncology

Cite this

Hashim, A., Rizzo, F., Marchese, G., Ravo, M., Tarallo, R., Nassa, G., ... Weisz, A. (2014). RNA sequencing identifies specific PIWI-interacting small noncoding RNA expression patterns in breast cancer. Oncotarget, 5(20), 9901-9910.

RNA sequencing identifies specific PIWI-interacting small noncoding RNA expression patterns in breast cancer. / Hashim, Adnan; Rizzo, Francesca; Marchese, Giovanna; Ravo, Maria; Tarallo, Roberta; Nassa, Giovanni; Giurato, Giorgio; Santamaria, Gianluca; Cordella, Angela; Cantarella, Concita; Weisz, Alessandro.

In: Oncotarget, Vol. 5, No. 20, 2014, p. 9901-9910.

Research output: Contribution to journalArticle

Hashim, A, Rizzo, F, Marchese, G, Ravo, M, Tarallo, R, Nassa, G, Giurato, G, Santamaria, G, Cordella, A, Cantarella, C & Weisz, A 2014, 'RNA sequencing identifies specific PIWI-interacting small noncoding RNA expression patterns in breast cancer', Oncotarget, vol. 5, no. 20, pp. 9901-9910.
Hashim A, Rizzo F, Marchese G, Ravo M, Tarallo R, Nassa G et al. RNA sequencing identifies specific PIWI-interacting small noncoding RNA expression patterns in breast cancer. Oncotarget. 2014;5(20):9901-9910.
Hashim, Adnan ; Rizzo, Francesca ; Marchese, Giovanna ; Ravo, Maria ; Tarallo, Roberta ; Nassa, Giovanni ; Giurato, Giorgio ; Santamaria, Gianluca ; Cordella, Angela ; Cantarella, Concita ; Weisz, Alessandro. / RNA sequencing identifies specific PIWI-interacting small noncoding RNA expression patterns in breast cancer. In: Oncotarget. 2014 ; Vol. 5, No. 20. pp. 9901-9910.
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