Abstract
PIWI-interacting small non-coding RNAs (piRNAs) are genetic and epigenetic regulatory factors in germline cells, where they maintain genome stability, are involved in RNA silencing and regulate gene expression. We found that the piRNA biogenesis and effector pathway are present in human breast cancer (BC) cells and, analyzing smallRNA-Seq data generated from BC cell lines and tumor biopsies, we identified >100 BC piRNAs, including some very abundant and/or differentially expressed in mammary epithelial compared to BC cells, where this was influenced by estrogen or estrogen receptor β, and in cancer respect to normal breast tissues. A search for mRNAs targeted by the BC piRNome revealed that eight piRNAs showing a specific expression pattern in breast tumors target key cancer cell pathways. Evidence of an active piRNA pathway in BC suggests that these small non-coding RNAs do exert transcriptional and post-transcriptional gene regulatory actions also in cancer cells.
| Original language | English |
|---|---|
| Pages (from-to) | 9901-9910 |
| Number of pages | 10 |
| Journal | Oncotarget |
| Volume | 5 |
| Issue number | 20 |
| Publication status | Published - 2014 |
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Keywords
- Breast cancer
- Estrogen receptor
- piRNAs
- PIWI
- RNA-sequencing
- Small non-coding RNA
ASJC Scopus subject areas
- Oncology
Cite this
RNA sequencing identifies specific PIWI-interacting small noncoding RNA expression patterns in breast cancer. / Hashim, Adnan; Rizzo, Francesca; Marchese, Giovanna; Ravo, Maria; Tarallo, Roberta; Nassa, Giovanni; Giurato, Giorgio; Santamaria, Gianluca; Cordella, Angela; Cantarella, Concita; Weisz, Alessandro.
In: Oncotarget, Vol. 5, No. 20, 2014, p. 9901-9910.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - RNA sequencing identifies specific PIWI-interacting small noncoding RNA expression patterns in breast cancer
AU - Hashim, Adnan
AU - Rizzo, Francesca
AU - Marchese, Giovanna
AU - Ravo, Maria
AU - Tarallo, Roberta
AU - Nassa, Giovanni
AU - Giurato, Giorgio
AU - Santamaria, Gianluca
AU - Cordella, Angela
AU - Cantarella, Concita
AU - Weisz, Alessandro
PY - 2014
Y1 - 2014
N2 - PIWI-interacting small non-coding RNAs (piRNAs) are genetic and epigenetic regulatory factors in germline cells, where they maintain genome stability, are involved in RNA silencing and regulate gene expression. We found that the piRNA biogenesis and effector pathway are present in human breast cancer (BC) cells and, analyzing smallRNA-Seq data generated from BC cell lines and tumor biopsies, we identified >100 BC piRNAs, including some very abundant and/or differentially expressed in mammary epithelial compared to BC cells, where this was influenced by estrogen or estrogen receptor β, and in cancer respect to normal breast tissues. A search for mRNAs targeted by the BC piRNome revealed that eight piRNAs showing a specific expression pattern in breast tumors target key cancer cell pathways. Evidence of an active piRNA pathway in BC suggests that these small non-coding RNAs do exert transcriptional and post-transcriptional gene regulatory actions also in cancer cells.
AB - PIWI-interacting small non-coding RNAs (piRNAs) are genetic and epigenetic regulatory factors in germline cells, where they maintain genome stability, are involved in RNA silencing and regulate gene expression. We found that the piRNA biogenesis and effector pathway are present in human breast cancer (BC) cells and, analyzing smallRNA-Seq data generated from BC cell lines and tumor biopsies, we identified >100 BC piRNAs, including some very abundant and/or differentially expressed in mammary epithelial compared to BC cells, where this was influenced by estrogen or estrogen receptor β, and in cancer respect to normal breast tissues. A search for mRNAs targeted by the BC piRNome revealed that eight piRNAs showing a specific expression pattern in breast tumors target key cancer cell pathways. Evidence of an active piRNA pathway in BC suggests that these small non-coding RNAs do exert transcriptional and post-transcriptional gene regulatory actions also in cancer cells.
KW - Breast cancer
KW - Estrogen receptor
KW - piRNAs
KW - PIWI
KW - RNA-sequencing
KW - Small non-coding RNA
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UR - http://www.scopus.com/inward/citedby.url?scp=84924934787&partnerID=8YFLogxK
M3 - Article
C2 - 25313140
AN - SCOPUS:84924934787
VL - 5
SP - 9901
EP - 9910
JO - Oncotarget
JF - Oncotarget
SN - 1949-2553
IS - 20
ER -