RNAIII-inhibiting peptide enhances healing of wounds infected with methicillin-resistant Staphylococcus aureus

Oriana Simonetti, Oscar Cirioni, Roberto Ghiselli, Gaia Goteri, Alessandro Scalise, Fiorenza Orlando, Carmela Silvestri, Alessandra Riva, Vittorio Saba, Kiran D. Madanahally, Annamaria Offidani, Naomi Balaban, Giorgio Scalise, Andrea Giacometti

Research output: Contribution to journalArticle

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Abstract

Quorum sensing is a mechanism through which a bacterial population receives input from neighboring cells and elicits an appropriate response to enable survival within the host. Inhibiting quorum sensing by RNAIII-inhibiting peptide (RIP) has been demonstrated as a very effective mode of prevention and therapy for device-associated staphylococcal infections and was tested here for healing of wounds that are otherwise resistant to conventional antibiotics. Wounds, established through the panniculus carnosus of BALB/c mice, were inoculated with 5 × 107 CFU of methicillin-resistant Staphylococcus aureus. Mice were treated with Allevyn, RIP-soaked Allevyn (containing 20 μg RIP), daily intraperitoneal teicoplanin (7 mg/kg of body weight), Allevyn and teicoplanin, and RIP-soaked Allevyn and daily intraperitoneal teicoplanin. The main outcome measures were quantitative bacterial culture and histological examination with assessment of microvessel density and of vascular endothelial growth factor (VEGF) expression in tissue sections. Treatment with RIP-soaked Allevyn together with teicoplanin injection greatly reduced the bacterial load to 13 CFU/g (control untreated animals had 108 CFU/g bacteria). All other treatments were also significantly effective but only reduced the bacterial load to about 103 CFU/ml. Histological examination indicated that only treatment with RIP-soaked Allevyn with teicoplanin injection restored epithelial, granulation, and collagen scores, as well as microvessel density and VEGF expression, to the levels found with uninfected mice. In conclusion, we observed that RIP may be useful for the management of infected wounds and that it could represent an exciting and future alternative to the conventional antibiotics, at present considered the gold-standard treatments for methicillin-resistant S. aureus infections.

Original languageEnglish
Pages (from-to)2205-2211
Number of pages7
JournalAntimicrobial Agents and Chemotherapy
Volume52
Issue number6
DOIs
Publication statusPublished - Jun 2008

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Methicillin-Resistant Staphylococcus aureus
Wound Healing
Teicoplanin
Quorum Sensing
Bacterial Load
Microvessels
Vascular Endothelial Growth Factor A
Anti-Bacterial Agents
Therapeutics
Staphylococcal Infections
Injections
Wounds and Injuries
RNAIII inhibiting peptide
Gold
allevyn
Collagen
Body Weight
Outcome Assessment (Health Care)
Bacteria
Equipment and Supplies

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

RNAIII-inhibiting peptide enhances healing of wounds infected with methicillin-resistant Staphylococcus aureus. / Simonetti, Oriana; Cirioni, Oscar; Ghiselli, Roberto; Goteri, Gaia; Scalise, Alessandro; Orlando, Fiorenza; Silvestri, Carmela; Riva, Alessandra; Saba, Vittorio; Madanahally, Kiran D.; Offidani, Annamaria; Balaban, Naomi; Scalise, Giorgio; Giacometti, Andrea.

In: Antimicrobial Agents and Chemotherapy, Vol. 52, No. 6, 06.2008, p. 2205-2211.

Research output: Contribution to journalArticle

Simonetti, O, Cirioni, O, Ghiselli, R, Goteri, G, Scalise, A, Orlando, F, Silvestri, C, Riva, A, Saba, V, Madanahally, KD, Offidani, A, Balaban, N, Scalise, G & Giacometti, A 2008, 'RNAIII-inhibiting peptide enhances healing of wounds infected with methicillin-resistant Staphylococcus aureus', Antimicrobial Agents and Chemotherapy, vol. 52, no. 6, pp. 2205-2211. https://doi.org/10.1128/AAC.01340-07
Simonetti, Oriana ; Cirioni, Oscar ; Ghiselli, Roberto ; Goteri, Gaia ; Scalise, Alessandro ; Orlando, Fiorenza ; Silvestri, Carmela ; Riva, Alessandra ; Saba, Vittorio ; Madanahally, Kiran D. ; Offidani, Annamaria ; Balaban, Naomi ; Scalise, Giorgio ; Giacometti, Andrea. / RNAIII-inhibiting peptide enhances healing of wounds infected with methicillin-resistant Staphylococcus aureus. In: Antimicrobial Agents and Chemotherapy. 2008 ; Vol. 52, No. 6. pp. 2205-2211.
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