Robertsonian metacentrics of the house mouse lose telomeric sequences but retain some minor satellite DNA in the pericentromeric area

Silvia Garagna, Dominique Broccoli, Carlo Alberto Redi, Jeremy B. Searle, Howard J. Cooke, Ernesto Capanna

Research output: Contribution to journalArticle

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Abstract

A combination of cytogenetic and molecular biology techniques were used to study the molecular composition and organisation of the pericentromeric regions of house mouse metacentric chromosomes, the products of Robertsonian (Rb) translocations between telocentrics. Regardless of whether mitotic or meiotic preparations were used, in situ hybridisation failed to reveal pericentromeric telomeric sequences on any of the Rb chromosomes, while all metacentrics retained detectable, although reduced (average 50 kb), amounts of minor satellite DNA in the vicinity of their centromeres. These results were supported by slot blot hybridisation which indicated that mice with 2 n=22 Rb chromosomes have 65% of telomeric sequences (which are allocated to the distal telomeres of both Rb and telocentric chromosomes and to the proximal telomeres of telocentrics) and 15% the amount of minor satellite, compared with mice with 2 n=40 all-telocentric chromosomes. Pulsed field gel electrophoresis and Southern analysis of DNA from Rb mice showed that the size of the telomeric arrays is similar to that of mice with all-telocentric chromosomes and that the minor satellite sequences were hybridising to larger fragments incorporating major satellite DNA. Since the telomeric sequences are closer to the physical end of the chromosome than the minor satellite sequences, the absence of telomeric sequences and the reduced amount of minor satellite sequences at the pericentromeric region of the Rb metacentrics suggest that the breakpoints for the Rb translocation occur very close to the minor satellite-major satellite border. Moreover, it is likely that the minor satellite is required for centromeric function, 50-67 kb being enough DNA to organise one centromere with a functionally active kinetochore.

Original languageEnglish
Pages (from-to)685-692
Number of pages8
JournalChromosoma
Volume103
Issue number10
DOIs
Publication statusPublished - Jul 1995

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Satellite DNA
Chromosomes
Centromere
Telomere
Kinetochores
Chromosomes, Human, Pair 22
Pulsed Field Gel Electrophoresis
DNA
Cytogenetics
In Situ Hybridization
Molecular Biology

ASJC Scopus subject areas

  • Genetics

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Robertsonian metacentrics of the house mouse lose telomeric sequences but retain some minor satellite DNA in the pericentromeric area. / Garagna, Silvia; Broccoli, Dominique; Redi, Carlo Alberto; Searle, Jeremy B.; Cooke, Howard J.; Capanna, Ernesto.

In: Chromosoma, Vol. 103, No. 10, 07.1995, p. 685-692.

Research output: Contribution to journalArticle

Garagna, Silvia ; Broccoli, Dominique ; Redi, Carlo Alberto ; Searle, Jeremy B. ; Cooke, Howard J. ; Capanna, Ernesto. / Robertsonian metacentrics of the house mouse lose telomeric sequences but retain some minor satellite DNA in the pericentromeric area. In: Chromosoma. 1995 ; Vol. 103, No. 10. pp. 685-692.
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