Role for p300 in Pax 8 induction of thyroperoxidase gene expression

Rossana De Leo, Stefania Miccadei, Enrico Zammarchi, Donato Civitareale

Research output: Contribution to journalArticle

Abstract

The nuclear p300 protein functions as a co-activator of gene transcription. Here we show that p300 works as a co-activator of the transcription factor Pax 8 on the thyroperoxidase gene promoter. Consistent with its role as co-activator, p300 potentiates Pax 8-activated transcription. Furthermore, we provide evidence supporting the formation of a complex between both factors in vivo and in vitro. This interaction involves the amino-terminal and CH3 domains of p300 and the trans-activation domain of Pax 8 at its carboxyl-terminal end. We show that the CH3 domain is crucial for the co-activator role of p300 on the thyroperoxidase gene promoter. In agreement with our finding and with the ability of the adenoviral protein E1A to bind p300, we show that EIA down-regulates Pax 8 activity.

Original languageEnglish
Pages (from-to)34100-34105
Number of pages6
JournalJournal of Biological Chemistry
Volume275
Issue number44
Publication statusPublished - Nov 3 2000

ASJC Scopus subject areas

  • Biochemistry

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    De Leo, R., Miccadei, S., Zammarchi, E., & Civitareale, D. (2000). Role for p300 in Pax 8 induction of thyroperoxidase gene expression. Journal of Biological Chemistry, 275(44), 34100-34105.