TY - JOUR
T1 - Role of β-defensin-1 polymorphisms in mother-to-child transmission of hiv-1
AU - Ricci, Elisabetta
AU - Malacrida, Sandro
AU - Zanchetta, Marisa
AU - Montagna, Marco
AU - Giaquinto, Carlo
AU - Rossi, Anita De
PY - 2009/5
Y1 - 2009/5
N2 - BACKGROUND AND OBJECTIVES: Mother-to-child transmission (MTCT) of HIV-1, the main source of pediatric AIDS, is multifactorial. Defensins provide microbial barriers and function as effectors of innate immunity. This study investigated the relationship between genetic variants of β-defensin-1 gene and MTCT of HIV-1. PATIENTS AND METHODS: Three hundred children, 118 HIV-1 infected and 182 HIV-1 uninfected, born to HIV-1-infected mothers who had not undergone antiretroviral therapy during pregnancy, and 84 HIV-1-infected mothers were analyzed. The single nucleotide polymorphisms -44C/G (rs1800972) and -52G/A (rs1799946) were genotyped by TaqMan allelic discrimination assay and sequencing. Statistical analyses were performed using SNPStats and Bonferroni correction for multiple tests. RESULTS: In children, the -52GG genotype and the -44G/-52G haplotype had a protective role against HIV-1 infection [odds ratio (OR) = 0.52, 95% confidence interval (CI) 0.31 to 0.86, P = 0.03 and OR = 0.50, 95% CI 0.31 to 0.83, P = 0.014, respectively]. In mothers, the -52GG genotype and the -44G/-52G haplotype were associated with low levels of HIV-1 plasma viremia (
AB - BACKGROUND AND OBJECTIVES: Mother-to-child transmission (MTCT) of HIV-1, the main source of pediatric AIDS, is multifactorial. Defensins provide microbial barriers and function as effectors of innate immunity. This study investigated the relationship between genetic variants of β-defensin-1 gene and MTCT of HIV-1. PATIENTS AND METHODS: Three hundred children, 118 HIV-1 infected and 182 HIV-1 uninfected, born to HIV-1-infected mothers who had not undergone antiretroviral therapy during pregnancy, and 84 HIV-1-infected mothers were analyzed. The single nucleotide polymorphisms -44C/G (rs1800972) and -52G/A (rs1799946) were genotyped by TaqMan allelic discrimination assay and sequencing. Statistical analyses were performed using SNPStats and Bonferroni correction for multiple tests. RESULTS: In children, the -52GG genotype and the -44G/-52G haplotype had a protective role against HIV-1 infection [odds ratio (OR) = 0.52, 95% confidence interval (CI) 0.31 to 0.86, P = 0.03 and OR = 0.50, 95% CI 0.31 to 0.83, P = 0.014, respectively]. In mothers, the -52GG genotype and the -44G/-52G haplotype were associated with low levels of HIV-1 plasma viremia (
KW - B-defensins
KW - HIV-1
KW - Mother-to-child transmission
KW - Natural immunity
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U2 - 10.1097/QAI.0b013e31819df249
DO - 10.1097/QAI.0b013e31819df249
M3 - Article
C2 - 19390326
AN - SCOPUS:67651173268
VL - 51
SP - 13
EP - 19
JO - Journal of Acquired Immune Deficiency Syndromes
JF - Journal of Acquired Immune Deficiency Syndromes
SN - 1525-4135
IS - 1
ER -