Role of 14-3-3 proteins in the regulation of neutral trehalase in the yeast Saccharomyces cerevisiae

Simona Panni, Christiane Landgraf, Rudolf Volkmer-Engert, Gianni Cesareni, Luisa Castagnoli

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In higher eukaryotes, 14-3-3 proteins participate in numerous cellular processes, and carry out their function through a variety of different molecular mechanisms, including regulation of protein localization and enzyme activation. Here, it is shown that the two yeast 14-3-3 homologues, Bmh1p and Bmh2p, form a complex with neutral trehalase (Nth1p), an enzyme that is responsible for trehalose degradation and is required in a variety of stress conditions. In a purified in vitro system, either one of the two 14-3-3 yeast isoforms are necessary for complete activation of neutral trehalase (Nth1p) after phosphorylation by PKA. It is further demonstrated that Bmh1p and Bmh2p bind to the amino-terminal region of phosphorylated trehalase, thereby modulating its enzymatic activity. This work represents the first demonstration of enzyme activation mediated by 14-3-3 binding in yeast.

Original languageEnglish
Pages (from-to)53-63
Number of pages11
JournalFEMS Yeast Research
Volume8
Issue number1
DOIs
Publication statusPublished - Feb 2008

Fingerprint

Trehalase
14-3-3 Proteins
Saccharomyces cerevisiae
Enzyme Activation
Yeasts
Trehalose
Eukaryota
Protein Isoforms
Phosphorylation
Enzymes
Proteins

Keywords

  • 14-3-3
  • Bmh1p
  • Bmh2p
  • Nth1p
  • Trehalose

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Infectious Diseases

Cite this

Role of 14-3-3 proteins in the regulation of neutral trehalase in the yeast Saccharomyces cerevisiae. / Panni, Simona; Landgraf, Christiane; Volkmer-Engert, Rudolf; Cesareni, Gianni; Castagnoli, Luisa.

In: FEMS Yeast Research, Vol. 8, No. 1, 02.2008, p. 53-63.

Research output: Contribution to journalArticle

Panni, Simona ; Landgraf, Christiane ; Volkmer-Engert, Rudolf ; Cesareni, Gianni ; Castagnoli, Luisa. / Role of 14-3-3 proteins in the regulation of neutral trehalase in the yeast Saccharomyces cerevisiae. In: FEMS Yeast Research. 2008 ; Vol. 8, No. 1. pp. 53-63.
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