Role of 5-HT receptors in the effect of d-fenfiuramine on gastric emptying and feeding behaviour as examined in the runway test

Rosario Samanin, Joanna Caroline Neill, Giuliano Grignaschi, Ines Martin Padura, Adalgisa Bizzi, Silvio Garattini

Research output: Contribution to journalArticle

Abstract

In one experiment, the effect of d-fenfluramine (DF) on gastric emptying was studied in rats treated i.p. with metergoline, a non-selective serotonin (5-HT) receptor antagonist, ritanserin, a selective 5-HT2 and 5-HT1C receptor antagonist, and xylamidine, a 5-HT antagonist which has poor access to the brain. Metergoline (1 mg/kg) but not ritanserin (0.5 mg/kg) or xylamidine (3 mg/kg) blocked the effect of 2.5 mg/kg DF studied 2 and 4 h after injection. In a second experiment, we studied the ability of metergoline to antagonise the effect of DF, administered after a meal, on runway performance, food intake and gastric emptying assessed 4 h later. Metergoline at a dose of 1 mg/kg did not antagonise the effect of DF (2.5 mg/kg) on runway performance but completely blocked the effect on gastric emptying. The data clearly show that DF delays gastric emptying by indirectly activating 5-HT1 receptors; this effect is not important for the ability of DF to reduce runway performance and food intake when the drug is injected after a pre-feeding period. While there is evidence that DF hastens the termination of the meal by a 5-HT mechanism, the data suggest that DF may prolong the satiating effect of food during the post-absorptive phase by mechanisms other than 5-HT.

Original languageEnglish
Pages (from-to)69-73
Number of pages5
JournalEuropean Journal of Pharmacology
Volume197
Issue number1-2
DOIs
Publication statusPublished - May 2 1991

Keywords

  • 5-HT
  • 5-HT receptors
  • 5-hydroxytryptamine
  • d-Fenfluramine
  • Gastric emptying
  • Runway performance
  • Satiety
  • serotonin

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

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