Role of ABC transporters in the BeWo trophoblast cell line

Monica Magnarin, Anna Rosati, Sara De Iudicibus, Fiora Bartoli, Giuliana Decorti

Research output: Contribution to journalArticle

Abstract

The transport of doxorubicin and rhodamine 123, substrates of ABC transporters, was evaluated in the BeWo stabilized trophoblast cell line. Both compounds were taken up by BeWo cells, but their intracellular concentrations were highly dependent on temperature, and significantly reduced at 4°C. The P-glycoprotein inhibitors verapamil and PSC833 did not modify the intracellular concentrations of the two substrates, suggesting therefore that, in these cells, the activity of P-glycoprotein is not important. MK571, which inhibits MRPs, was on the contrary effective in increasing rhodamine 123 intracellular concentrations. The efflux of both fluorescent substrates was extremely slow, and slightly reduced by MK571. Finally, a polarized transport of doxorubicin from basal to apical side was evident, although only during the first 60 min of incubation, and was reduced by P-glycoprotein, MRP, and BCRP inhibitors. No MDR1 expression was revealed at the mRNA and protein levels; on the contrary, MRP1 and BCRP were expressed in these cells. In BeWo cells the activity of ABC transporters, and in particular of P-glycoprotein, seems to be extremely limited.

Original languageEnglish
Pages (from-to)763-769
Number of pages7
JournalToxicology Mechanisms and Methods
Volume18
Issue number9
DOIs
Publication statusPublished - Nov 2008

Fingerprint

ATP-Binding Cassette Transporters
Trophoblasts
P-Glycoprotein
Cells
Rhodamine 123
Cell Line
Doxorubicin
Substrates
Verapamil
Messenger RNA
Temperature
Proteins

Keywords

  • BeWo cells
  • Multidrug resistance-related proteins
  • Multidrug transporters
  • P-glycoprotein
  • Placenta

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Magnarin, M., Rosati, A., De Iudicibus, S., Bartoli, F., & Decorti, G. (2008). Role of ABC transporters in the BeWo trophoblast cell line. Toxicology Mechanisms and Methods, 18(9), 763-769. https://doi.org/10.1080/15376510802428591

Role of ABC transporters in the BeWo trophoblast cell line. / Magnarin, Monica; Rosati, Anna; De Iudicibus, Sara; Bartoli, Fiora; Decorti, Giuliana.

In: Toxicology Mechanisms and Methods, Vol. 18, No. 9, 11.2008, p. 763-769.

Research output: Contribution to journalArticle

Magnarin, M, Rosati, A, De Iudicibus, S, Bartoli, F & Decorti, G 2008, 'Role of ABC transporters in the BeWo trophoblast cell line', Toxicology Mechanisms and Methods, vol. 18, no. 9, pp. 763-769. https://doi.org/10.1080/15376510802428591
Magnarin M, Rosati A, De Iudicibus S, Bartoli F, Decorti G. Role of ABC transporters in the BeWo trophoblast cell line. Toxicology Mechanisms and Methods. 2008 Nov;18(9):763-769. https://doi.org/10.1080/15376510802428591
Magnarin, Monica ; Rosati, Anna ; De Iudicibus, Sara ; Bartoli, Fiora ; Decorti, Giuliana. / Role of ABC transporters in the BeWo trophoblast cell line. In: Toxicology Mechanisms and Methods. 2008 ; Vol. 18, No. 9. pp. 763-769.
@article{3f1bf50a6e724e3b99a007be68cb3d9b,
title = "Role of ABC transporters in the BeWo trophoblast cell line",
abstract = "The transport of doxorubicin and rhodamine 123, substrates of ABC transporters, was evaluated in the BeWo stabilized trophoblast cell line. Both compounds were taken up by BeWo cells, but their intracellular concentrations were highly dependent on temperature, and significantly reduced at 4°C. The P-glycoprotein inhibitors verapamil and PSC833 did not modify the intracellular concentrations of the two substrates, suggesting therefore that, in these cells, the activity of P-glycoprotein is not important. MK571, which inhibits MRPs, was on the contrary effective in increasing rhodamine 123 intracellular concentrations. The efflux of both fluorescent substrates was extremely slow, and slightly reduced by MK571. Finally, a polarized transport of doxorubicin from basal to apical side was evident, although only during the first 60 min of incubation, and was reduced by P-glycoprotein, MRP, and BCRP inhibitors. No MDR1 expression was revealed at the mRNA and protein levels; on the contrary, MRP1 and BCRP were expressed in these cells. In BeWo cells the activity of ABC transporters, and in particular of P-glycoprotein, seems to be extremely limited.",
keywords = "BeWo cells, Multidrug resistance-related proteins, Multidrug transporters, P-glycoprotein, Placenta",
author = "Monica Magnarin and Anna Rosati and {De Iudicibus}, Sara and Fiora Bartoli and Giuliana Decorti",
year = "2008",
month = "11",
doi = "10.1080/15376510802428591",
language = "English",
volume = "18",
pages = "763--769",
journal = "Toxicology Mechanisms and Methods",
issn = "1537-6516",
publisher = "Informa Healthcare",
number = "9",

}

TY - JOUR

T1 - Role of ABC transporters in the BeWo trophoblast cell line

AU - Magnarin, Monica

AU - Rosati, Anna

AU - De Iudicibus, Sara

AU - Bartoli, Fiora

AU - Decorti, Giuliana

PY - 2008/11

Y1 - 2008/11

N2 - The transport of doxorubicin and rhodamine 123, substrates of ABC transporters, was evaluated in the BeWo stabilized trophoblast cell line. Both compounds were taken up by BeWo cells, but their intracellular concentrations were highly dependent on temperature, and significantly reduced at 4°C. The P-glycoprotein inhibitors verapamil and PSC833 did not modify the intracellular concentrations of the two substrates, suggesting therefore that, in these cells, the activity of P-glycoprotein is not important. MK571, which inhibits MRPs, was on the contrary effective in increasing rhodamine 123 intracellular concentrations. The efflux of both fluorescent substrates was extremely slow, and slightly reduced by MK571. Finally, a polarized transport of doxorubicin from basal to apical side was evident, although only during the first 60 min of incubation, and was reduced by P-glycoprotein, MRP, and BCRP inhibitors. No MDR1 expression was revealed at the mRNA and protein levels; on the contrary, MRP1 and BCRP were expressed in these cells. In BeWo cells the activity of ABC transporters, and in particular of P-glycoprotein, seems to be extremely limited.

AB - The transport of doxorubicin and rhodamine 123, substrates of ABC transporters, was evaluated in the BeWo stabilized trophoblast cell line. Both compounds were taken up by BeWo cells, but their intracellular concentrations were highly dependent on temperature, and significantly reduced at 4°C. The P-glycoprotein inhibitors verapamil and PSC833 did not modify the intracellular concentrations of the two substrates, suggesting therefore that, in these cells, the activity of P-glycoprotein is not important. MK571, which inhibits MRPs, was on the contrary effective in increasing rhodamine 123 intracellular concentrations. The efflux of both fluorescent substrates was extremely slow, and slightly reduced by MK571. Finally, a polarized transport of doxorubicin from basal to apical side was evident, although only during the first 60 min of incubation, and was reduced by P-glycoprotein, MRP, and BCRP inhibitors. No MDR1 expression was revealed at the mRNA and protein levels; on the contrary, MRP1 and BCRP were expressed in these cells. In BeWo cells the activity of ABC transporters, and in particular of P-glycoprotein, seems to be extremely limited.

KW - BeWo cells

KW - Multidrug resistance-related proteins

KW - Multidrug transporters

KW - P-glycoprotein

KW - Placenta

UR - http://www.scopus.com/inward/record.url?scp=57349141471&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57349141471&partnerID=8YFLogxK

U2 - 10.1080/15376510802428591

DO - 10.1080/15376510802428591

M3 - Article

C2 - 20020937

AN - SCOPUS:57349141471

VL - 18

SP - 763

EP - 769

JO - Toxicology Mechanisms and Methods

JF - Toxicology Mechanisms and Methods

SN - 1537-6516

IS - 9

ER -