Role of accessory cells in the induction of a secondary cytotoxic response to Moloney murine sarcoma virus-induced tumors

G. Biasi, L. Saggin, F. Dazzi, A. De Rossi, D. Collavo

Research output: Contribution to journalArticle

Abstract

The role of Ia-positive accessory cells in the generation of a secondary cytotoxic response to tumor-associated antigens induced by Moloney murine sarcoma virus (M-MSV) was evaluated. Spleen cells from M-MSV-immune A.TL mice, depleted of accessory cells by anti-Ia(k) serum plus C treatment and stimulated in secondary mixed leukocyte tumor cell culture (MLTC) with syngeneic Ia-negative A6ATL Moloney leukemic cells, failed to generate virus-specific cytotoxic T lymphocytes (CTL). CTL generation in Ia-depleted MLTC may be reconstituted by the addition of nonimmune Ia-positive spleen or peritoneal cells obtained not only from syngeneic A.TL but also from I-incompatible A.TH mice. This lack of restriction observed in accessory cell function is explained in terms of a nonspecific mechanism of CTL triggering mediated by soluble factors. In fact, IL 2 as well as supernatants obtained from I region-incompatible cultures consisting of M-MSV-immune, Ia-depleted A.TL spleen cells and A.TH Ia-positive cells, reconstituted secondary virus-specific CTL generation.

Original languageEnglish
Pages (from-to)1447-1450
Number of pages4
JournalJournal of Immunology
Volume130
Issue number3
Publication statusPublished - 1983

ASJC Scopus subject areas

  • Immunology

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