Role of acetate during platelet storage in a synthetic medium

F. Bertolini, S. Murphy, P. Rebulla, G. Sirchia

Research output: Contribution to journalArticle

Abstract

It has previously been shown that buffy coat platelet concentrates (BC- PCs) stored in a medium made up of approximately 70 percent platelet storage medium (Plasma-Lyte A, PL) and 30 percent plasma (BC-PC-P) are effective in vivo after 9 to 12 days of storage. In addition to sodium, potassium, magnesium, and chloride, PL contains 27 mM (27 mmol/L) sodium acetate and 23 mM (23 mmol/L) sodium gluconate. This study investigated the effect of acetate and gluconate on platelet metabolism. Identical BC-PCs were stored at 22 ± 2°C in PL (BC-PC-P); PL with gluconate but without acetate, termed PL- A (BC-PC-A); or PL with acetate but without gluconate, termed PL-G (BC-PC-G). On Day 1 of storage, no significant differences were seen between the three groups of BC-PCs. In both BC-PC-P and BC-PC-G, pH and bicarbonate were stable at 7.0 ± 0.03 and 8.4 ± 0.9 mEq per L throughout 10 days of storage, whereas in BC-PC-A, they fell to 6.7 ± 0.05 and 5.5 ± 0.8 mEq per L on Day 5 (p <0.01 vs. Day 1) and to 6.1 ± 0.1 and 1.2 ± 0.4 mEq per L, respectively, on Day 10. The buffering capacities of 70 percent PL, PL-A, or PL-G and 30 percent plasma were similar in a platelet-free setting when incremental additions of lactic acid were made. The role of acetate was further studied by adding 14C- or 3H-labeled acetate to BC-PC-P. On Day 7 of storage, radioactivity decreased to 79 ± 1 percent of baseline in 14C- labeled BC-PC-P (p = 0.02), while it was unchanged in 3H-labeled BC-PC-P and in control samples in which 14C- and 3H-acetate were added to platelet- free plasma or PL. These studies indicate that platelets metabolize acetate to CO2 during storage and that acetate metabolism plays a major role in pH control during storage of BC-PCs.

Original languageEnglish
Pages (from-to)152-156
Number of pages5
JournalTransfusion
Volume32
Issue number2
Publication statusPublished - 1992

Fingerprint

Acetates
Blood Platelets
Plasmalyte A
Sodium Acetate
Magnesium Chloride
Potassium Chloride
Bicarbonates
Sodium Chloride
Radioactivity
Lactic Acid
gluconic acid

ASJC Scopus subject areas

  • Immunology
  • Hematology

Cite this

Role of acetate during platelet storage in a synthetic medium. / Bertolini, F.; Murphy, S.; Rebulla, P.; Sirchia, G.

In: Transfusion, Vol. 32, No. 2, 1992, p. 152-156.

Research output: Contribution to journalArticle

Bertolini, F, Murphy, S, Rebulla, P & Sirchia, G 1992, 'Role of acetate during platelet storage in a synthetic medium', Transfusion, vol. 32, no. 2, pp. 152-156.
Bertolini, F. ; Murphy, S. ; Rebulla, P. ; Sirchia, G. / Role of acetate during platelet storage in a synthetic medium. In: Transfusion. 1992 ; Vol. 32, No. 2. pp. 152-156.
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abstract = "It has previously been shown that buffy coat platelet concentrates (BC- PCs) stored in a medium made up of approximately 70 percent platelet storage medium (Plasma-Lyte A, PL) and 30 percent plasma (BC-PC-P) are effective in vivo after 9 to 12 days of storage. In addition to sodium, potassium, magnesium, and chloride, PL contains 27 mM (27 mmol/L) sodium acetate and 23 mM (23 mmol/L) sodium gluconate. This study investigated the effect of acetate and gluconate on platelet metabolism. Identical BC-PCs were stored at 22 ± 2°C in PL (BC-PC-P); PL with gluconate but without acetate, termed PL- A (BC-PC-A); or PL with acetate but without gluconate, termed PL-G (BC-PC-G). On Day 1 of storage, no significant differences were seen between the three groups of BC-PCs. In both BC-PC-P and BC-PC-G, pH and bicarbonate were stable at 7.0 ± 0.03 and 8.4 ± 0.9 mEq per L throughout 10 days of storage, whereas in BC-PC-A, they fell to 6.7 ± 0.05 and 5.5 ± 0.8 mEq per L on Day 5 (p <0.01 vs. Day 1) and to 6.1 ± 0.1 and 1.2 ± 0.4 mEq per L, respectively, on Day 10. The buffering capacities of 70 percent PL, PL-A, or PL-G and 30 percent plasma were similar in a platelet-free setting when incremental additions of lactic acid were made. The role of acetate was further studied by adding 14C- or 3H-labeled acetate to BC-PC-P. On Day 7 of storage, radioactivity decreased to 79 ± 1 percent of baseline in 14C- labeled BC-PC-P (p = 0.02), while it was unchanged in 3H-labeled BC-PC-P and in control samples in which 14C- and 3H-acetate were added to platelet- free plasma or PL. These studies indicate that platelets metabolize acetate to CO2 during storage and that acetate metabolism plays a major role in pH control during storage of BC-PCs.",
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N2 - It has previously been shown that buffy coat platelet concentrates (BC- PCs) stored in a medium made up of approximately 70 percent platelet storage medium (Plasma-Lyte A, PL) and 30 percent plasma (BC-PC-P) are effective in vivo after 9 to 12 days of storage. In addition to sodium, potassium, magnesium, and chloride, PL contains 27 mM (27 mmol/L) sodium acetate and 23 mM (23 mmol/L) sodium gluconate. This study investigated the effect of acetate and gluconate on platelet metabolism. Identical BC-PCs were stored at 22 ± 2°C in PL (BC-PC-P); PL with gluconate but without acetate, termed PL- A (BC-PC-A); or PL with acetate but without gluconate, termed PL-G (BC-PC-G). On Day 1 of storage, no significant differences were seen between the three groups of BC-PCs. In both BC-PC-P and BC-PC-G, pH and bicarbonate were stable at 7.0 ± 0.03 and 8.4 ± 0.9 mEq per L throughout 10 days of storage, whereas in BC-PC-A, they fell to 6.7 ± 0.05 and 5.5 ± 0.8 mEq per L on Day 5 (p <0.01 vs. Day 1) and to 6.1 ± 0.1 and 1.2 ± 0.4 mEq per L, respectively, on Day 10. The buffering capacities of 70 percent PL, PL-A, or PL-G and 30 percent plasma were similar in a platelet-free setting when incremental additions of lactic acid were made. The role of acetate was further studied by adding 14C- or 3H-labeled acetate to BC-PC-P. On Day 7 of storage, radioactivity decreased to 79 ± 1 percent of baseline in 14C- labeled BC-PC-P (p = 0.02), while it was unchanged in 3H-labeled BC-PC-P and in control samples in which 14C- and 3H-acetate were added to platelet- free plasma or PL. These studies indicate that platelets metabolize acetate to CO2 during storage and that acetate metabolism plays a major role in pH control during storage of BC-PCs.

AB - It has previously been shown that buffy coat platelet concentrates (BC- PCs) stored in a medium made up of approximately 70 percent platelet storage medium (Plasma-Lyte A, PL) and 30 percent plasma (BC-PC-P) are effective in vivo after 9 to 12 days of storage. In addition to sodium, potassium, magnesium, and chloride, PL contains 27 mM (27 mmol/L) sodium acetate and 23 mM (23 mmol/L) sodium gluconate. This study investigated the effect of acetate and gluconate on platelet metabolism. Identical BC-PCs were stored at 22 ± 2°C in PL (BC-PC-P); PL with gluconate but without acetate, termed PL- A (BC-PC-A); or PL with acetate but without gluconate, termed PL-G (BC-PC-G). On Day 1 of storage, no significant differences were seen between the three groups of BC-PCs. In both BC-PC-P and BC-PC-G, pH and bicarbonate were stable at 7.0 ± 0.03 and 8.4 ± 0.9 mEq per L throughout 10 days of storage, whereas in BC-PC-A, they fell to 6.7 ± 0.05 and 5.5 ± 0.8 mEq per L on Day 5 (p <0.01 vs. Day 1) and to 6.1 ± 0.1 and 1.2 ± 0.4 mEq per L, respectively, on Day 10. The buffering capacities of 70 percent PL, PL-A, or PL-G and 30 percent plasma were similar in a platelet-free setting when incremental additions of lactic acid were made. The role of acetate was further studied by adding 14C- or 3H-labeled acetate to BC-PC-P. On Day 7 of storage, radioactivity decreased to 79 ± 1 percent of baseline in 14C- labeled BC-PC-P (p = 0.02), while it was unchanged in 3H-labeled BC-PC-P and in control samples in which 14C- and 3H-acetate were added to platelet- free plasma or PL. These studies indicate that platelets metabolize acetate to CO2 during storage and that acetate metabolism plays a major role in pH control during storage of BC-PCs.

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