Role of alpha-synuclein in autophagy modulation of primary human T lymphocytes

T. Colasanti, M. Vomero, C. Alessandri, C. Barbati, A. Maselli, C. Camperio, F. Conti, A. Tinari, C. Carlo-Stella, L. Tuosto, D. Benincasa, G. Valesini, W. Malorni, M. Pierdominici, E. Ortona

Research output: Contribution to journalArticle

Abstract

It has been demonstrated that α-synuclein can aggregate and contribute to the pathogenesis of some neurodegenerative diseases and it is capable of hindering autophagy in neuronal cells. Here, we investigated the implication of α-synuclein in the autophagy process in primary human T lymphocytes. We provide evidence that: (i) knocking down of the α-synuclein gene resulted in increased autophagy, (ii) autophagy induction by energy deprivation was associated with a significant decrease of α-synuclein levels, (iii) autophagy inhibition by 3-methyladenine or by ATG5 knocking down led to a significant increase of α-synuclein levels, and (iv) autophagy impairment, constitutive in T lymphocytes from patients with systemic lupus erythematosus, was associated with abnormal accumulation of α-synuclein aggregates. These results suggest that α-synuclein could be considered as an autophagy-related marker of peripheral blood lymphocytes, potentially suitable for use in the clinical practice.

Original languageEnglish
Article numbere1265
JournalCell Death and Disease
Volume5
DOIs
Publication statusPublished - May 29 2014

Fingerprint

Synucleins
alpha-Synuclein
Autophagy
T-Lymphocytes
Neurodegenerative Diseases
Systemic Lupus Erythematosus
Lymphocytes

Keywords

  • autophagy
  • lymphoma
  • systemic lupus erythematosus
  • T lymphocytes
  • α-synuclein

ASJC Scopus subject areas

  • Cell Biology
  • Immunology
  • Cancer Research
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Colasanti, T., Vomero, M., Alessandri, C., Barbati, C., Maselli, A., Camperio, C., ... Ortona, E. (2014). Role of alpha-synuclein in autophagy modulation of primary human T lymphocytes. Cell Death and Disease, 5, [e1265]. https://doi.org/10.1038/cddis.2014.211

Role of alpha-synuclein in autophagy modulation of primary human T lymphocytes. / Colasanti, T.; Vomero, M.; Alessandri, C.; Barbati, C.; Maselli, A.; Camperio, C.; Conti, F.; Tinari, A.; Carlo-Stella, C.; Tuosto, L.; Benincasa, D.; Valesini, G.; Malorni, W.; Pierdominici, M.; Ortona, E.

In: Cell Death and Disease, Vol. 5, e1265, 29.05.2014.

Research output: Contribution to journalArticle

Colasanti, T, Vomero, M, Alessandri, C, Barbati, C, Maselli, A, Camperio, C, Conti, F, Tinari, A, Carlo-Stella, C, Tuosto, L, Benincasa, D, Valesini, G, Malorni, W, Pierdominici, M & Ortona, E 2014, 'Role of alpha-synuclein in autophagy modulation of primary human T lymphocytes', Cell Death and Disease, vol. 5, e1265. https://doi.org/10.1038/cddis.2014.211
Colasanti T, Vomero M, Alessandri C, Barbati C, Maselli A, Camperio C et al. Role of alpha-synuclein in autophagy modulation of primary human T lymphocytes. Cell Death and Disease. 2014 May 29;5. e1265. https://doi.org/10.1038/cddis.2014.211
Colasanti, T. ; Vomero, M. ; Alessandri, C. ; Barbati, C. ; Maselli, A. ; Camperio, C. ; Conti, F. ; Tinari, A. ; Carlo-Stella, C. ; Tuosto, L. ; Benincasa, D. ; Valesini, G. ; Malorni, W. ; Pierdominici, M. ; Ortona, E. / Role of alpha-synuclein in autophagy modulation of primary human T lymphocytes. In: Cell Death and Disease. 2014 ; Vol. 5.
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