TY - JOUR
T1 - Role of alpha-synuclein in autophagy modulation of primary human T lymphocytes
AU - Colasanti, T.
AU - Vomero, M.
AU - Alessandri, C.
AU - Barbati, C.
AU - Maselli, A.
AU - Camperio, C.
AU - Conti, F.
AU - Tinari, A.
AU - Carlo-Stella, C.
AU - Tuosto, L.
AU - Benincasa, D.
AU - Valesini, G.
AU - Malorni, W.
AU - Pierdominici, M.
AU - Ortona, E.
PY - 2014/5/29
Y1 - 2014/5/29
N2 - It has been demonstrated that α-synuclein can aggregate and contribute to the pathogenesis of some neurodegenerative diseases and it is capable of hindering autophagy in neuronal cells. Here, we investigated the implication of α-synuclein in the autophagy process in primary human T lymphocytes. We provide evidence that: (i) knocking down of the α-synuclein gene resulted in increased autophagy, (ii) autophagy induction by energy deprivation was associated with a significant decrease of α-synuclein levels, (iii) autophagy inhibition by 3-methyladenine or by ATG5 knocking down led to a significant increase of α-synuclein levels, and (iv) autophagy impairment, constitutive in T lymphocytes from patients with systemic lupus erythematosus, was associated with abnormal accumulation of α-synuclein aggregates. These results suggest that α-synuclein could be considered as an autophagy-related marker of peripheral blood lymphocytes, potentially suitable for use in the clinical practice.
AB - It has been demonstrated that α-synuclein can aggregate and contribute to the pathogenesis of some neurodegenerative diseases and it is capable of hindering autophagy in neuronal cells. Here, we investigated the implication of α-synuclein in the autophagy process in primary human T lymphocytes. We provide evidence that: (i) knocking down of the α-synuclein gene resulted in increased autophagy, (ii) autophagy induction by energy deprivation was associated with a significant decrease of α-synuclein levels, (iii) autophagy inhibition by 3-methyladenine or by ATG5 knocking down led to a significant increase of α-synuclein levels, and (iv) autophagy impairment, constitutive in T lymphocytes from patients with systemic lupus erythematosus, was associated with abnormal accumulation of α-synuclein aggregates. These results suggest that α-synuclein could be considered as an autophagy-related marker of peripheral blood lymphocytes, potentially suitable for use in the clinical practice.
KW - autophagy
KW - lymphoma
KW - systemic lupus erythematosus
KW - T lymphocytes
KW - α-synuclein
UR - http://www.scopus.com/inward/record.url?scp=84924249398&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84924249398&partnerID=8YFLogxK
U2 - 10.1038/cddis.2014.211
DO - 10.1038/cddis.2014.211
M3 - Article
C2 - 24874737
AN - SCOPUS:84924249398
VL - 5
JO - Cell Death and Disease
JF - Cell Death and Disease
SN - 2041-4889
M1 - e1265
ER -