Abstract
The chick embryo chorioallantoic membrane (CAM) is supplied by an extensive capillary network. We have previously demonstrated that a Mr 16,000 basic fibroblast growth factor (FGF2)-like molecule is present in the CAM. At present, no data are available on the cellular source(s) of FGF2 in the CAM. In this work, CAM has been investigated by in situ hybridization with the aim to identify the source(s) of endogenous FGF2 during development. The immunohistochemical expression of fibronectin, laminin and type IV collagen in the CAM extracellular matrix (ECM) and the ultrastructural relationships between chorionic epithelium and the underlying capillary plexus were also studied. Our findings strongly suggest that FGF2 regulates the development of the capillary plexus by two sequential steps. In an early paracrine phase, chorionic epithelial cells secrete FGF2, thus eliciting an angiogenic response in the undifferentiated mesodermal blood vessels. In response to this paracrine signalling, the newly formed endothelial cells move through a permissive ECM and migrate beneath the chorion. Here, they synthesize an autocrine supply of FGF2 necessary to further proliferate and differentiate, thus originating the capillary plexus.
| Original language | English |
|---|---|
| Pages (from-to) | 127-136 |
| Number of pages | 10 |
| Journal | Journal of Submicroscopic Cytology and Pathology |
| Volume | 30 |
| Issue number | 1 |
| Publication status | Published - Jan 1998 |
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Keywords
- Angiogenesis
- Basic fibroblast growth factor
- Chick embryo
- Chorioallantoic membrane
ASJC Scopus subject areas
- Cell Biology
- Histology
- Pathology and Forensic Medicine
Cite this
Role of basic fibroblast growth factor in the formation of the capillary plexus in the chick embryo chorioallantoic membrane. An in situ hybridization, immunohistochemical and ultrastructural study. / Ribatti, D.; Bertossi, M.; Nico, B.; Vacca, A.; Ria, R.; Riva, A.; Roncali, L.; Presta, M.
In: Journal of Submicroscopic Cytology and Pathology, Vol. 30, No. 1, 01.1998, p. 127-136.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Role of basic fibroblast growth factor in the formation of the capillary plexus in the chick embryo chorioallantoic membrane. An in situ hybridization, immunohistochemical and ultrastructural study
AU - Ribatti, D.
AU - Bertossi, M.
AU - Nico, B.
AU - Vacca, A.
AU - Ria, R.
AU - Riva, A.
AU - Roncali, L.
AU - Presta, M.
PY - 1998/1
Y1 - 1998/1
N2 - The chick embryo chorioallantoic membrane (CAM) is supplied by an extensive capillary network. We have previously demonstrated that a Mr 16,000 basic fibroblast growth factor (FGF2)-like molecule is present in the CAM. At present, no data are available on the cellular source(s) of FGF2 in the CAM. In this work, CAM has been investigated by in situ hybridization with the aim to identify the source(s) of endogenous FGF2 during development. The immunohistochemical expression of fibronectin, laminin and type IV collagen in the CAM extracellular matrix (ECM) and the ultrastructural relationships between chorionic epithelium and the underlying capillary plexus were also studied. Our findings strongly suggest that FGF2 regulates the development of the capillary plexus by two sequential steps. In an early paracrine phase, chorionic epithelial cells secrete FGF2, thus eliciting an angiogenic response in the undifferentiated mesodermal blood vessels. In response to this paracrine signalling, the newly formed endothelial cells move through a permissive ECM and migrate beneath the chorion. Here, they synthesize an autocrine supply of FGF2 necessary to further proliferate and differentiate, thus originating the capillary plexus.
AB - The chick embryo chorioallantoic membrane (CAM) is supplied by an extensive capillary network. We have previously demonstrated that a Mr 16,000 basic fibroblast growth factor (FGF2)-like molecule is present in the CAM. At present, no data are available on the cellular source(s) of FGF2 in the CAM. In this work, CAM has been investigated by in situ hybridization with the aim to identify the source(s) of endogenous FGF2 during development. The immunohistochemical expression of fibronectin, laminin and type IV collagen in the CAM extracellular matrix (ECM) and the ultrastructural relationships between chorionic epithelium and the underlying capillary plexus were also studied. Our findings strongly suggest that FGF2 regulates the development of the capillary plexus by two sequential steps. In an early paracrine phase, chorionic epithelial cells secrete FGF2, thus eliciting an angiogenic response in the undifferentiated mesodermal blood vessels. In response to this paracrine signalling, the newly formed endothelial cells move through a permissive ECM and migrate beneath the chorion. Here, they synthesize an autocrine supply of FGF2 necessary to further proliferate and differentiate, thus originating the capillary plexus.
KW - Angiogenesis
KW - Basic fibroblast growth factor
KW - Chick embryo
KW - Chorioallantoic membrane
UR - http://www.scopus.com/inward/record.url?scp=0031614074&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031614074&partnerID=8YFLogxK
M3 - Article
C2 - 9530860
AN - SCOPUS:0031614074
VL - 30
SP - 127
EP - 136
JO - Journal of Submicroscopic Cytology and Pathology
JF - Journal of Submicroscopic Cytology and Pathology
SN - 1122-9497
IS - 1
ER -