Role of basic fibroblast growth factor in the formation of the capillary plexus in the chick embryo chorioallantoic membrane. An in situ hybridization, immunohistochemical and ultrastructural study

D. Ribatti, M. Bertossi, B. Nico, A. Vacca, R. Ria, A. Riva, L. Roncali, M. Presta

Research output: Contribution to journalArticle

Abstract

The chick embryo chorioallantoic membrane (CAM) is supplied by an extensive capillary network. We have previously demonstrated that a Mr 16,000 basic fibroblast growth factor (FGF2)-like molecule is present in the CAM. At present, no data are available on the cellular source(s) of FGF2 in the CAM. In this work, CAM has been investigated by in situ hybridization with the aim to identify the source(s) of endogenous FGF2 during development. The immunohistochemical expression of fibronectin, laminin and type IV collagen in the CAM extracellular matrix (ECM) and the ultrastructural relationships between chorionic epithelium and the underlying capillary plexus were also studied. Our findings strongly suggest that FGF2 regulates the development of the capillary plexus by two sequential steps. In an early paracrine phase, chorionic epithelial cells secrete FGF2, thus eliciting an angiogenic response in the undifferentiated mesodermal blood vessels. In response to this paracrine signalling, the newly formed endothelial cells move through a permissive ECM and migrate beneath the chorion. Here, they synthesize an autocrine supply of FGF2 necessary to further proliferate and differentiate, thus originating the capillary plexus.

Original languageEnglish
Pages (from-to)127-136
Number of pages10
JournalJournal of Submicroscopic Cytology and Pathology
Volume30
Issue number1
Publication statusPublished - Jan 1998

Fingerprint

Chorioallantoic Membrane
Fibroblast Growth Factor 2
Chick Embryo
In Situ Hybridization
Extracellular Matrix
Paracrine Communication
Chorion
Collagen Type IV
Laminin
Fibronectins
Blood Vessels
Epithelium
Endothelial Cells
Epithelial Cells

Keywords

  • Angiogenesis
  • Basic fibroblast growth factor
  • Chick embryo
  • Chorioallantoic membrane

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Pathology and Forensic Medicine

Cite this

Role of basic fibroblast growth factor in the formation of the capillary plexus in the chick embryo chorioallantoic membrane. An in situ hybridization, immunohistochemical and ultrastructural study. / Ribatti, D.; Bertossi, M.; Nico, B.; Vacca, A.; Ria, R.; Riva, A.; Roncali, L.; Presta, M.

In: Journal of Submicroscopic Cytology and Pathology, Vol. 30, No. 1, 01.1998, p. 127-136.

Research output: Contribution to journalArticle

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