Ruolo della migrazione cellulare nella patogenesi dell'artrite reumatoide: studi in vivo in topi SCID trapiantati con membrana sinoviale umana.

Translated title of the contribution: Role of cell migration in the pathogenesis of rheumatoid arthritis: in vivo studies in SCID mice transplanted with human synovial membrane

F. Ingegnoli, M. Blades, A. Manzo, S. Wahid, M. Perretti, G. Panayi, C. Pitzalis

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

OBJECTIVE: Adhesion mechanisms play a central role in the recruitment of leukocytes which characteristically infiltrate rheumatoid synovium. Therefore, we adapted an animal model, in which human rheumatoid synovium was transplanted into severe combined immunodeficient (SCID) mice, to study the effects of Tumor Necrosis Factor-alpha (TNF-alpha) in modulatine leukocyte migration and to investigate the chemotactic potential of Stromal Derived Factor-1 alpha (SDF-1 alpha). MATERIALS AND METHODS: Human synovium samples, obtained from patients undergoing joint replacement, were divided into two parts. One was analysed by immunohistology and the other was implanted subcutaneously into SCID mice under general anaesthesia. Four weeks post-transplantation, grafts were injected with optimal dose of SDF-1, TNF-alpha or saline (negative control). At the same time, animals were injected iv with fluorescently labelled cells. 48 hours later mice were sacrificed and grafts removed for cryo-hystology. The number of cells migrating to the grafts was determined by UV-microscopy and the results expressed as cells per high power field. RESULTS AND CONCLUSIONS: In these studies we provide the evidence that: 1) the animal model, in which human tissues are grafted into SCID mice, can be used to study cell migration under controlled experimental conditions; 2) direct intragraft injection of TNF-alpha increases lymphocytes migration and up-regulates the expression of human adhesion molecules (CAMs) and 3) SDF-1 alphainjected intragraft increases the migration of the pro-myelo-monocytic U937 cells to synovial transplants, even more efficiently than TNF-alpha, but without modifications of CAMs' expression.

Original languageItalian
Pages (from-to)128-132
Number of pages5
JournalReumatismo
Volume54
Issue number2
Publication statusPublished - Apr 2002

Fingerprint

SCID Mice
Synovial Membrane
Cell Movement
Rheumatoid Arthritis
Tumor Necrosis Factor-alpha
Transplants
Replacement Arthroplasties
Leukocytes
Animal Models
U937 Cells
General Anesthesia
Microscopy
Up-Regulation
Cell Count
Transplantation
Lymphocytes
Injections

ASJC Scopus subject areas

  • Rheumatology

Cite this

Ingegnoli, F., Blades, M., Manzo, A., Wahid, S., Perretti, M., Panayi, G., & Pitzalis, C. (2002). Ruolo della migrazione cellulare nella patogenesi dell'artrite reumatoide: studi in vivo in topi SCID trapiantati con membrana sinoviale umana. Reumatismo, 54(2), 128-132.

Ruolo della migrazione cellulare nella patogenesi dell'artrite reumatoide : studi in vivo in topi SCID trapiantati con membrana sinoviale umana. / Ingegnoli, F.; Blades, M.; Manzo, A.; Wahid, S.; Perretti, M.; Panayi, G.; Pitzalis, C.

In: Reumatismo, Vol. 54, No. 2, 04.2002, p. 128-132.

Research output: Contribution to journalArticle

Ingegnoli, F, Blades, M, Manzo, A, Wahid, S, Perretti, M, Panayi, G & Pitzalis, C 2002, 'Ruolo della migrazione cellulare nella patogenesi dell'artrite reumatoide: studi in vivo in topi SCID trapiantati con membrana sinoviale umana.', Reumatismo, vol. 54, no. 2, pp. 128-132.
Ingegnoli, F. ; Blades, M. ; Manzo, A. ; Wahid, S. ; Perretti, M. ; Panayi, G. ; Pitzalis, C. / Ruolo della migrazione cellulare nella patogenesi dell'artrite reumatoide : studi in vivo in topi SCID trapiantati con membrana sinoviale umana. In: Reumatismo. 2002 ; Vol. 54, No. 2. pp. 128-132.
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