Role of erythropoietin in the angiogenic activity of bone marrow endothelial cells of MGUS and multiple myeloma patients

Aurelia Lamanuzzi, Ilaria Saltarella, Arianna Ferrucci, Roberto Ria, Simona Ruggieri, Vito Racanelli, Luigia Rao, Tiziana Annese, Beatrice Nico, Angelo Vacca, Domenico Ribatti

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Increasing evidences suggest several biological roles for erythropoietin and its receptor (Epo and EpoR), unrelated to erythropoiesis, including angiogenesis. Here, we detected the expression of EpoR in bone marrow-derived endothelial cells from monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM) patients (MGECs and MMECs, respectively) and assessed whether Epo plays a role in MGECs- and MMECs-mediated angiogenesis. We show that EpoR is expressed by both MGECs and MMECs even though at a higher level in the first ones. Both EC types respond to rHuEpo in terms of cell proliferation, whereas other responses, including activation of JAK2/STAT5 and PI3K/Akt pathways, cell migration and capillarogenesis are enhanced by Epo in MGECs, but not in MMECs. In addition, the conditioned media of both Epo-treated cells induce a strong angiogenic response in vivo in the chorioallantoic membrane assay, comparable to that of vascular endothelial growth factor (VEGF). Overall, these data highlight the effect of Epo on MGECs- and MMECs-mediated angiogenesis: MGECs are more responsive to Epo treatment than MMECs, probably because over-angiogenic phenotype of MMECs is already activated by their autocrine/paracrine loops occurring in the "angiogenic switch" from MGUS.

Original languageEnglish
Pages (from-to)14510-14521
Number of pages12
JournalOncotarget
Volume7
Issue number12
DOIs
Publication statusPublished - Mar 22 2016

Fingerprint

Monoclonal Gammopathy of Undetermined Significance
Erythropoietin
Multiple Myeloma
Bone Marrow Cells
Endothelial Cells
Erythropoietin Receptors
Chorioallantoic Membrane
Erythropoiesis
Conditioned Culture Medium
Phosphatidylinositol 3-Kinases
Vascular Endothelial Growth Factor A
Cell Movement
Bone Marrow
Cell Proliferation
Phenotype
Therapeutics

Keywords

  • Angiogenesis
  • Endothelial cells
  • Erythropoietin
  • Monoclonal gammopathy of undetermined significance
  • Multiple myeloma

ASJC Scopus subject areas

  • Oncology

Cite this

Lamanuzzi, A., Saltarella, I., Ferrucci, A., Ria, R., Ruggieri, S., Racanelli, V., ... Ribatti, D. (2016). Role of erythropoietin in the angiogenic activity of bone marrow endothelial cells of MGUS and multiple myeloma patients. Oncotarget, 7(12), 14510-14521. https://doi.org/10.18632/oncotarget.7587

Role of erythropoietin in the angiogenic activity of bone marrow endothelial cells of MGUS and multiple myeloma patients. / Lamanuzzi, Aurelia; Saltarella, Ilaria; Ferrucci, Arianna; Ria, Roberto; Ruggieri, Simona; Racanelli, Vito; Rao, Luigia; Annese, Tiziana; Nico, Beatrice; Vacca, Angelo; Ribatti, Domenico.

In: Oncotarget, Vol. 7, No. 12, 22.03.2016, p. 14510-14521.

Research output: Contribution to journalArticle

Lamanuzzi, A, Saltarella, I, Ferrucci, A, Ria, R, Ruggieri, S, Racanelli, V, Rao, L, Annese, T, Nico, B, Vacca, A & Ribatti, D 2016, 'Role of erythropoietin in the angiogenic activity of bone marrow endothelial cells of MGUS and multiple myeloma patients', Oncotarget, vol. 7, no. 12, pp. 14510-14521. https://doi.org/10.18632/oncotarget.7587
Lamanuzzi, Aurelia ; Saltarella, Ilaria ; Ferrucci, Arianna ; Ria, Roberto ; Ruggieri, Simona ; Racanelli, Vito ; Rao, Luigia ; Annese, Tiziana ; Nico, Beatrice ; Vacca, Angelo ; Ribatti, Domenico. / Role of erythropoietin in the angiogenic activity of bone marrow endothelial cells of MGUS and multiple myeloma patients. In: Oncotarget. 2016 ; Vol. 7, No. 12. pp. 14510-14521.
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