Role of FOXA in mitochondrial citrate carrier gene expression and insulin secretion

Vito Iacobazzi, Vittoria Infantino, Faustino Bisaccia, Alessandra Castegna, Ferdinando Palmieri

Research output: Contribution to journalArticle

Abstract

In this study, we have investigated the transcriptional role of the FOXA site present in the promoter of the mitochondrial citrate carrier (CIC) gene. We have shown that wild-type (but not mutated) CIC FOXA site cloned in front of the luciferase promoter confers transcriptional activation of the gene reporter, particularly in cells overexpressing FOXA1. We have also demonstrated that overexpression and silencing of FOXA increases and reduces CIC transcript and protein levels, respectively. In addition, FOXA1 silencing in INS-1 cells decreases not only CIC mRNA and protein but also the amount of citrate in the cytosol and glucose-stimulated insulin secretion. These results show that FOXA plays a role in the transcriptional regulation of CIC and in insulin secretion.

Original languageEnglish
Pages (from-to)220-224
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume385
Issue number2
DOIs
Publication statusPublished - Jul 24 2009

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Keywords

  • Citrate carrier
  • FOXA
  • Gene expression
  • Insulin secretion
  • Mitochondria
  • Promoter
  • Transcription

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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