TY - JOUR
T1 - Role of FOXA in mitochondrial citrate carrier gene expression and insulin secretion
AU - Iacobazzi, Vito
AU - Infantino, Vittoria
AU - Bisaccia, Faustino
AU - Castegna, Alessandra
AU - Palmieri, Ferdinando
PY - 2009/7/24
Y1 - 2009/7/24
N2 - In this study, we have investigated the transcriptional role of the FOXA site present in the promoter of the mitochondrial citrate carrier (CIC) gene. We have shown that wild-type (but not mutated) CIC FOXA site cloned in front of the luciferase promoter confers transcriptional activation of the gene reporter, particularly in cells overexpressing FOXA1. We have also demonstrated that overexpression and silencing of FOXA increases and reduces CIC transcript and protein levels, respectively. In addition, FOXA1 silencing in INS-1 cells decreases not only CIC mRNA and protein but also the amount of citrate in the cytosol and glucose-stimulated insulin secretion. These results show that FOXA plays a role in the transcriptional regulation of CIC and in insulin secretion.
AB - In this study, we have investigated the transcriptional role of the FOXA site present in the promoter of the mitochondrial citrate carrier (CIC) gene. We have shown that wild-type (but not mutated) CIC FOXA site cloned in front of the luciferase promoter confers transcriptional activation of the gene reporter, particularly in cells overexpressing FOXA1. We have also demonstrated that overexpression and silencing of FOXA increases and reduces CIC transcript and protein levels, respectively. In addition, FOXA1 silencing in INS-1 cells decreases not only CIC mRNA and protein but also the amount of citrate in the cytosol and glucose-stimulated insulin secretion. These results show that FOXA plays a role in the transcriptional regulation of CIC and in insulin secretion.
KW - Citrate carrier
KW - FOXA
KW - Gene expression
KW - Insulin secretion
KW - Mitochondria
KW - Promoter
KW - Transcription
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U2 - 10.1016/j.bbrc.2009.05.030
DO - 10.1016/j.bbrc.2009.05.030
M3 - Article
C2 - 19445897
AN - SCOPUS:67349127497
VL - 385
SP - 220
EP - 224
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -