Role of FOXA in mitochondrial citrate carrier gene expression and insulin secretion

Vito Iacobazzi; Vittoria Infantino; Faustino Bisaccia; Alessandra Castegna; Ferdinando Palmieri

doi:10.1016/j.bbrc.2009.05.030

Role of FOXA in mitochondrial citrate carrier gene expression and insulin secretion

Vito Iacobazzi, Vittoria Infantino, Faustino Bisaccia, Alessandra Castegna, Ferdinando Palmieri

IRCCS Giovanni Paolo II

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, we have investigated the transcriptional role of the FOXA site present in the promoter of the mitochondrial citrate carrier (CIC) gene. We have shown that wild-type (but not mutated) CIC FOXA site cloned in front of the luciferase promoter confers transcriptional activation of the gene reporter, particularly in cells overexpressing FOXA1. We have also demonstrated that overexpression and silencing of FOXA increases and reduces CIC transcript and protein levels, respectively. In addition, FOXA1 silencing in INS-1 cells decreases not only CIC mRNA and protein but also the amount of citrate in the cytosol and glucose-stimulated insulin secretion. These results show that FOXA plays a role in the transcriptional regulation of CIC and in insulin secretion.

Original language	English
Pages (from-to)	220-224
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	385
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2009.05.030
Publication status	Published - Jul 24 2009

Keywords

Citrate carrier
FOXA
Gene expression
Insulin secretion
Mitochondria
Promoter
Transcription

ASJC Scopus subject areas

Biochemistry
Biophysics
Cell Biology
Molecular Biology

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10.1016/j.bbrc.2009.05.030

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abstract = "In this study, we have investigated the transcriptional role of the FOXA site present in the promoter of the mitochondrial citrate carrier (CIC) gene. We have shown that wild-type (but not mutated) CIC FOXA site cloned in front of the luciferase promoter confers transcriptional activation of the gene reporter, particularly in cells overexpressing FOXA1. We have also demonstrated that overexpression and silencing of FOXA increases and reduces CIC transcript and protein levels, respectively. In addition, FOXA1 silencing in INS-1 cells decreases not only CIC mRNA and protein but also the amount of citrate in the cytosol and glucose-stimulated insulin secretion. These results show that FOXA plays a role in the transcriptional regulation of CIC and in insulin secretion.",

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AU - Iacobazzi, Vito

AU - Infantino, Vittoria

AU - Bisaccia, Faustino

AU - Castegna, Alessandra

AU - Palmieri, Ferdinando

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KW - Citrate carrier

KW - FOXA

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KW - Insulin secretion

KW - Mitochondria

KW - Promoter

KW - Transcription

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Role of FOXA in mitochondrial citrate carrier gene expression and insulin secretion

Abstract

Keywords

ASJC Scopus subject areas

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