Role of genes for normal growth factors in human malignancy.

S. A. Aaronson, H. Igarashi, C. D. Rao, E. Finzi, T. P. Fleming, O. Segatto, K. C. Robbins

Research output: Contribution to journalArticle

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Abstract

The human homologue of the v-sis oncogene encodes one chain of human platelet-derived growth factor (PDGF-2). Previous studies have shown that expression of the coding sequence for this growth factor induces malignant transformation of NIH/3T3 cells. We demonstrate the detection of sis/PDGF-2 products indistinguishable from functional PDGF-2 homodimers in human tumor cells. These findings support the concept that expression of the sis/PDGF-2 product in human cells responsive to its proliferative actions can be an important step in the processes leading to malignancy. Unlike sis/PDGF-2, which remains tightly cell-associated, another growth factor, termed transforming growth factor alpha (TGF alpha), is actively secreted. Expression vectors for the TGF alpha coding sequence failed to induce primary transformed foci upon transfection of NIH/3T3 cells despite high levels of TGF alpha synthesized by these cells. Moreover, transfectants selected for secretion of high levels of TGF alpha failed to form colonies on contact inhibited NIH/3T3 monolayers or to induce tumors upon inoculation of nude mice. Thus, the ability of a growth factor to induce autonomous in vitro or in vivo growth is dependent upon more than expression of cognate receptors by the cell in which it is synthesized.

Original languageEnglish
Pages (from-to)95-108
Number of pages14
JournalPrincess Takamatsu symposia
Volume17
Publication statusPublished - 1986

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Transforming Growth Factor alpha
Intercellular Signaling Peptides and Proteins
NIH 3T3 Cells
Genes
Neoplasms
sis Genes
Platelet-Derived Growth Factor
Nude Mice
Transfection
Growth

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Aaronson, S. A., Igarashi, H., Rao, C. D., Finzi, E., Fleming, T. P., Segatto, O., & Robbins, K. C. (1986). Role of genes for normal growth factors in human malignancy. Princess Takamatsu symposia, 17, 95-108.

Role of genes for normal growth factors in human malignancy. / Aaronson, S. A.; Igarashi, H.; Rao, C. D.; Finzi, E.; Fleming, T. P.; Segatto, O.; Robbins, K. C.

In: Princess Takamatsu symposia, Vol. 17, 1986, p. 95-108.

Research output: Contribution to journalArticle

Aaronson, SA, Igarashi, H, Rao, CD, Finzi, E, Fleming, TP, Segatto, O & Robbins, KC 1986, 'Role of genes for normal growth factors in human malignancy.', Princess Takamatsu symposia, vol. 17, pp. 95-108.
Aaronson SA, Igarashi H, Rao CD, Finzi E, Fleming TP, Segatto O et al. Role of genes for normal growth factors in human malignancy. Princess Takamatsu symposia. 1986;17:95-108.
Aaronson, S. A. ; Igarashi, H. ; Rao, C. D. ; Finzi, E. ; Fleming, T. P. ; Segatto, O. ; Robbins, K. C. / Role of genes for normal growth factors in human malignancy. In: Princess Takamatsu symposia. 1986 ; Vol. 17. pp. 95-108.
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