Role of Hog1 and Yaf9 in the transcriptional response of Saccharomyces cerevisiae to cesium chloride

Valerio Del Vescovo, Viviana Casagrande, Michele M. Bianchi, Eugenia Piccinni, Laura Frontali, Cristina Militti, Vivienne Fardeau, Frédéric Devaux, Claudio Di Sanza, Carlo Presutti, Rodolfo Negri

Research output: Contribution to journalArticle

Abstract

We analyzed the global transcriptional response of Saccharomyces cerevisiae cells exposed to different concentrations of CsCl in the growth medium and at different times after addition. Early responsive genes were mainly involved in cell wall structure and biosynthesis. About half of the induced genes were previously shown to respond to other alkali metal cations in a Hog1-dependent fashion. Western blot analysis confirmed that cesium concentrations as low as 100 mM activate Hog1 phosphorylation. Another important fraction of the cesium-modulated genes requires Yaf9p for full responsiveness as shown by the transcriptome of a yaf9-deleted strain in the presence of cesium. We showed that a cell wall-restructuring process promptly occurs in response to cesium addition, which is dependent on the presence of both Hog1 and Yaf9 proteins. Moreover, the sensitivity to low concentration of cesium of the yaf9-deleted strain is not observed in a strain carrying the hog1/yaf9 double deletion. We conclude that the observed early transcriptional modulation of cell wall genes has a crucial role in S. cerevisiae adaptation to cesium.

Original languageEnglish
Pages (from-to)110-120
Number of pages11
JournalPhysiol Genomics
Volume33
Issue number1
DOIs
Publication statusPublished - Mar 14 2008

Fingerprint

Cesium
Saccharomyces cerevisiae
Cell Wall
Genes
Alkali Metals
Transcriptome
cesium chloride
Cations
Western Blotting
Phosphorylation
Growth
Proteins

Keywords

  • Alkali metal cations
  • Caesium
  • Cell wall
  • HOG1
  • YAF9

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

Del Vescovo, V., Casagrande, V., Bianchi, M. M., Piccinni, E., Frontali, L., Militti, C., ... Negri, R. (2008). Role of Hog1 and Yaf9 in the transcriptional response of Saccharomyces cerevisiae to cesium chloride. Physiol Genomics, 33(1), 110-120. https://doi.org/10.1152/physiolgenomics.00251.2007

Role of Hog1 and Yaf9 in the transcriptional response of Saccharomyces cerevisiae to cesium chloride. / Del Vescovo, Valerio; Casagrande, Viviana; Bianchi, Michele M.; Piccinni, Eugenia; Frontali, Laura; Militti, Cristina; Fardeau, Vivienne; Devaux, Frédéric; Di Sanza, Claudio; Presutti, Carlo; Negri, Rodolfo.

In: Physiol Genomics, Vol. 33, No. 1, 14.03.2008, p. 110-120.

Research output: Contribution to journalArticle

Del Vescovo, V, Casagrande, V, Bianchi, MM, Piccinni, E, Frontali, L, Militti, C, Fardeau, V, Devaux, F, Di Sanza, C, Presutti, C & Negri, R 2008, 'Role of Hog1 and Yaf9 in the transcriptional response of Saccharomyces cerevisiae to cesium chloride', Physiol Genomics, vol. 33, no. 1, pp. 110-120. https://doi.org/10.1152/physiolgenomics.00251.2007
Del Vescovo V, Casagrande V, Bianchi MM, Piccinni E, Frontali L, Militti C et al. Role of Hog1 and Yaf9 in the transcriptional response of Saccharomyces cerevisiae to cesium chloride. Physiol Genomics. 2008 Mar 14;33(1):110-120. https://doi.org/10.1152/physiolgenomics.00251.2007
Del Vescovo, Valerio ; Casagrande, Viviana ; Bianchi, Michele M. ; Piccinni, Eugenia ; Frontali, Laura ; Militti, Cristina ; Fardeau, Vivienne ; Devaux, Frédéric ; Di Sanza, Claudio ; Presutti, Carlo ; Negri, Rodolfo. / Role of Hog1 and Yaf9 in the transcriptional response of Saccharomyces cerevisiae to cesium chloride. In: Physiol Genomics. 2008 ; Vol. 33, No. 1. pp. 110-120.
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