Role of human serum and cellular factors on the growth of peripheral blood CFU-gm [published erratum to be found in Int J Cell Cloning 1988 Jan;6(1): 80]

G. Bellone, P. Lista, F. Veglia, L. Pegoraro, L. Lista

Research output: Contribution to journalArticle

Abstract

The effects of fetal calf serum (FCS), autologous serum (AS) and pooled human serum (PS) on granulocyte-macrophage progenitors (CFU-gm) from normal human peripheral blood mononuclear non-adherent cells (MNAC) were studied to determine optimal growth conditions. PS provided fewer variations and better growth conditions than AS and FCS. Moreover, serum inhibitors affecting both autologous and heterologous CFU-gm were detected in a small group (10%) of donors. Since CFU-gm from different donors display a relatively uniform cell-cycle status and membrane phenotype, under optimal growth conditions the number of CFU-gm probably reflects the size of the circulating myeloid progenitor cell compartment.

Original languageEnglish
Pages (from-to)463-471
Number of pages9
JournalInternational Journal of Cell Cloning
Volume5
Issue number6
Publication statusPublished - Nov 1987

ASJC Scopus subject areas

  • Cell Biology

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