Role of iron and ferritin in TNFα-induced apoptosis in HeLa cells

Anna Cozzi; Sonia Levi; Barbara Corsi; Paolo Santambrogio; Alessandro Campanella; Gianmario Gerardi; Paolo Arosio

doi:10.1016/S0014-5793(03)00114-5

Role of iron and ferritin in TNFα-induced apoptosis in HeLa cells

Anna Cozzi, Sonia Levi, Barbara Corsi, Paolo Santambrogio, Alessandro Campanella, Gianmario Gerardi, Paolo Arosio

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

We found that tumor necrosis factor α (TNFα)-induced apoptosis in HeLa cells was accompanied by a ∼2-fold increase in H- and L-ferritin and a decrease in transferrin receptor, two indices of increased iron availability. Iron supplementation and overexpression of H-ferritin or its mutant with an inactivated ferroxidase center reduced by about ∼50% the number of apoptotic cells after TNFα-treatment, while overexpression of L-ferritin was ineffective. The data indicate that H-ferritin has an anti-apoptotic activity unrelated to its ferroxidase activity and to its capacity to modify cellular iron metabolism.

Original language	English
Pages (from-to)	187-192
Number of pages	6
Journal	FEBS Letters
Volume	537
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(03)00114-5
Publication status	Published - Feb 27 2003

Keywords

Ferritin
Iron metabolism
Iron regulatory protein
Oxidative damage

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/S0014-5793(03)00114-5

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abstract = "We found that tumor necrosis factor α (TNFα)-induced apoptosis in HeLa cells was accompanied by a ∼2-fold increase in H- and L-ferritin and a decrease in transferrin receptor, two indices of increased iron availability. Iron supplementation and overexpression of H-ferritin or its mutant with an inactivated ferroxidase center reduced by about ∼50% the number of apoptotic cells after TNFα-treatment, while overexpression of L-ferritin was ineffective. The data indicate that H-ferritin has an anti-apoptotic activity unrelated to its ferroxidase activity and to its capacity to modify cellular iron metabolism.",

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AU - Cozzi, Anna

AU - Levi, Sonia

AU - Corsi, Barbara

AU - Santambrogio, Paolo

AU - Campanella, Alessandro

AU - Gerardi, Gianmario

AU - Arosio, Paolo

PY - 2003/2/27

Y1 - 2003/2/27

N2 - We found that tumor necrosis factor α (TNFα)-induced apoptosis in HeLa cells was accompanied by a ∼2-fold increase in H- and L-ferritin and a decrease in transferrin receptor, two indices of increased iron availability. Iron supplementation and overexpression of H-ferritin or its mutant with an inactivated ferroxidase center reduced by about ∼50% the number of apoptotic cells after TNFα-treatment, while overexpression of L-ferritin was ineffective. The data indicate that H-ferritin has an anti-apoptotic activity unrelated to its ferroxidase activity and to its capacity to modify cellular iron metabolism.

AB - We found that tumor necrosis factor α (TNFα)-induced apoptosis in HeLa cells was accompanied by a ∼2-fold increase in H- and L-ferritin and a decrease in transferrin receptor, two indices of increased iron availability. Iron supplementation and overexpression of H-ferritin or its mutant with an inactivated ferroxidase center reduced by about ∼50% the number of apoptotic cells after TNFα-treatment, while overexpression of L-ferritin was ineffective. The data indicate that H-ferritin has an anti-apoptotic activity unrelated to its ferroxidase activity and to its capacity to modify cellular iron metabolism.

KW - Ferritin

KW - Iron metabolism

KW - Iron regulatory protein

KW - Oxidative damage

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Role of iron and ferritin in TNFα-induced apoptosis in HeLa cells

Abstract

Keywords

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