Abstract
We found that tumor necrosis factor α (TNFα)-induced apoptosis in HeLa cells was accompanied by a ∼2-fold increase in H- and L-ferritin and a decrease in transferrin receptor, two indices of increased iron availability. Iron supplementation and overexpression of H-ferritin or its mutant with an inactivated ferroxidase center reduced by about ∼50% the number of apoptotic cells after TNFα-treatment, while overexpression of L-ferritin was ineffective. The data indicate that H-ferritin has an anti-apoptotic activity unrelated to its ferroxidase activity and to its capacity to modify cellular iron metabolism.
| Original language | English |
|---|---|
| Pages (from-to) | 187-192 |
| Number of pages | 6 |
| Journal | FEBS Letters |
| Volume | 537 |
| Issue number | 1-3 |
| DOIs | |
| Publication status | Published - Feb 27 2003 |
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Keywords
- Ferritin
- Iron metabolism
- Iron regulatory protein
- Oxidative damage
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Role of iron and ferritin in TNFα-induced apoptosis in HeLa cells. / Cozzi, Anna; Levi, Sonia; Corsi, Barbara; Santambrogio, Paolo; Campanella, Alessandro; Gerardi, Gianmario; Arosio, Paolo.
In: FEBS Letters, Vol. 537, No. 1-3, 27.02.2003, p. 187-192.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Role of iron and ferritin in TNFα-induced apoptosis in HeLa cells
AU - Cozzi, Anna
AU - Levi, Sonia
AU - Corsi, Barbara
AU - Santambrogio, Paolo
AU - Campanella, Alessandro
AU - Gerardi, Gianmario
AU - Arosio, Paolo
PY - 2003/2/27
Y1 - 2003/2/27
N2 - We found that tumor necrosis factor α (TNFα)-induced apoptosis in HeLa cells was accompanied by a ∼2-fold increase in H- and L-ferritin and a decrease in transferrin receptor, two indices of increased iron availability. Iron supplementation and overexpression of H-ferritin or its mutant with an inactivated ferroxidase center reduced by about ∼50% the number of apoptotic cells after TNFα-treatment, while overexpression of L-ferritin was ineffective. The data indicate that H-ferritin has an anti-apoptotic activity unrelated to its ferroxidase activity and to its capacity to modify cellular iron metabolism.
AB - We found that tumor necrosis factor α (TNFα)-induced apoptosis in HeLa cells was accompanied by a ∼2-fold increase in H- and L-ferritin and a decrease in transferrin receptor, two indices of increased iron availability. Iron supplementation and overexpression of H-ferritin or its mutant with an inactivated ferroxidase center reduced by about ∼50% the number of apoptotic cells after TNFα-treatment, while overexpression of L-ferritin was ineffective. The data indicate that H-ferritin has an anti-apoptotic activity unrelated to its ferroxidase activity and to its capacity to modify cellular iron metabolism.
KW - Ferritin
KW - Iron metabolism
KW - Iron regulatory protein
KW - Oxidative damage
UR - http://www.scopus.com/inward/record.url?scp=0037468397&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037468397&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(03)00114-5
DO - 10.1016/S0014-5793(03)00114-5
M3 - Article
VL - 537
SP - 187
EP - 192
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-3
ER -