Role of iron and ferritin in TNFα-induced apoptosis in HeLa cells

Anna Cozzi, Sonia Levi, Barbara Corsi, Paolo Santambrogio, Alessandro Campanella, Gianmario Gerardi, Paolo Arosio

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

We found that tumor necrosis factor α (TNFα)-induced apoptosis in HeLa cells was accompanied by a ∼2-fold increase in H- and L-ferritin and a decrease in transferrin receptor, two indices of increased iron availability. Iron supplementation and overexpression of H-ferritin or its mutant with an inactivated ferroxidase center reduced by about ∼50% the number of apoptotic cells after TNFα-treatment, while overexpression of L-ferritin was ineffective. The data indicate that H-ferritin has an anti-apoptotic activity unrelated to its ferroxidase activity and to its capacity to modify cellular iron metabolism.

Original languageEnglish
Pages (from-to)187-192
Number of pages6
JournalFEBS Letters
Volume537
Issue number1-3
DOIs
Publication statusPublished - Feb 27 2003

Fingerprint

Apoferritins
Ferritins
HeLa Cells
Iron
Tumor Necrosis Factor-alpha
Apoptosis
Ceruloplasmin
Transferrin Receptors
Metabolism
Cell Count
Cells
Availability

Keywords

  • Ferritin
  • Iron metabolism
  • Iron regulatory protein
  • Oxidative damage

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Role of iron and ferritin in TNFα-induced apoptosis in HeLa cells. / Cozzi, Anna; Levi, Sonia; Corsi, Barbara; Santambrogio, Paolo; Campanella, Alessandro; Gerardi, Gianmario; Arosio, Paolo.

In: FEBS Letters, Vol. 537, No. 1-3, 27.02.2003, p. 187-192.

Research output: Contribution to journalArticle

Cozzi, Anna ; Levi, Sonia ; Corsi, Barbara ; Santambrogio, Paolo ; Campanella, Alessandro ; Gerardi, Gianmario ; Arosio, Paolo. / Role of iron and ferritin in TNFα-induced apoptosis in HeLa cells. In: FEBS Letters. 2003 ; Vol. 537, No. 1-3. pp. 187-192.
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