Role of L-type Ca2+ channels in neural stem/progenitor cell differentiation

Marcello D'Ascenzo, Roberto Piacentini, Patrizia Casalbore, Manuela Budoni, Roberto Pallini, Gian Battista Azzena, Claudio Grassi

Research output: Contribution to journalArticle


Ca2+ influx through voltage-gated Ca2+ channels, especially the L-type (Cav1), activates downstream signaling to the nucleus that affects gene expression and, consequently, cell fate. We hypothesized that these Ca2+ signals may also influence the neuronal differentiation of neural stem/progenitor cells (NSCs) derived from the brain cortex of postnatal mice. We first studied Ca2+ transients induced by membrane depolarization in Fluo 4-AM-loaded NSCs using confocal microscopy. Undifferentiated cells (nestin+) exhibited no detectable Ca 2+ signals whereas, during 12 days of fetal bovine serum-induced differentiation, neurons (β-III-tubulin+/MAP2+) displayed time-dependent increases in intracellular Ca2+ transients, with ΔF/F ratios ranging from 0.4 on day 3 to 3.3 on day 12. Patch-clamp experiments revealed similar correlation between NSC differentiation and macroscopic Ba2+ current density. These currents were markedly reduced (-77%) by Cav1 channel blockade with 5 μm nifedipine. To determine the influence of Cav1-mediated Ca2+ influx on NSC differentiation, cells were cultured in differentiative medium with either nifedipine (5 μm) or the L-channel activator Bay K 8644 (10 μm). The latter treatment significantly increased the percentage of β-III- tubulin+/MAP2+ cells whereas nifedipine produced opposite effects. Pretreatment with nifedipine also inhibited the functional maturation of neurons, which responded to membrane depolarization with weak Ca2+ signals. Conversely, Bay K 8644 pretreatment significantly enhanced the percentage of responsive cells and the amplitudes of Ca2+ transients. These data suggest that NSC differentiation is strongly correlated with the expression of voltage-gated Ca2+ channels, especially the Ca v1, and that Ca2+ influx through these channels plays a key role in promoting neuronal differentiation.

Original languageEnglish
Pages (from-to)935-944
Number of pages10
JournalEuropean Journal of Neuroscience
Issue number4
Publication statusPublished - Feb 2006



  • Confocal Ca imaging
  • Mouse brain cortex
  • Neurogenesis
  • Patch-clamp recordings
  • Voltage-gated Ca channels

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

D'Ascenzo, M., Piacentini, R., Casalbore, P., Budoni, M., Pallini, R., Azzena, G. B., & Grassi, C. (2006). Role of L-type Ca2+ channels in neural stem/progenitor cell differentiation. European Journal of Neuroscience, 23(4), 935-944.