Role of leucocyte procoagulant activity in endotoxin-induced DIC

Evidence from comparative studies in rats and rabbits

Nicola Semeraro, Mario Colucci, Luciana Mussoni, Maria B. Donati

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We have investigated the ability of rat and rabbit leucocytes to generate coagulant activity (PCA) in response to endotoxin in vitro and in vivo. On prolonged incubation with endotoxin (10 μg/ml f.c.) isolated rabbit leucocytes developed strong PCA as measured by clotting and amidolytic assay. In constrast, rat leucocytes failed to produce any PCA even in the presence of huge amounts of endotoxin (200 μg/ml f.c.). When rabbits were given two spaced endotoxin injections (25 μg/kg b.w., 24 h apart) blood leucocytes harvested 30-60 min after the second injection consistently showed marked PCA. Again, unlike the rabbit, rat leucocytes obtained after two endotoxin injections (up 2 mg/kg b.w.) were completely devoid of PCA. These findings support the view that leucocytes are involved in endotoxin-induced disseminated intravascular coagulation in rabbits. On the other hand the poor response of rat leucocytes to endotoxin might help explain the resistance of rats to DIC and Sanarelli-Shwartzman reaction.

Original languageEnglish
Pages (from-to)646-648
Number of pages3
JournalAgents and Actions
Volume11
Issue number6-7
DOIs
Publication statusPublished - 1981

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Dacarbazine
Endotoxins
Passive Cutaneous Anaphylaxis
Rats
Leukocytes
Rabbits
Injections
Shwartzman Phenomenon
Coagulants
Disseminated Intravascular Coagulation
leukocyte procoagulant activity
Coagulation
Assays
Blood

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Toxicology

Cite this

Role of leucocyte procoagulant activity in endotoxin-induced DIC : Evidence from comparative studies in rats and rabbits. / Semeraro, Nicola; Colucci, Mario; Mussoni, Luciana; Donati, Maria B.

In: Agents and Actions, Vol. 11, No. 6-7, 1981, p. 646-648.

Research output: Contribution to journalArticle

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