Abstract
The presented study was designed to characterize the adhesive interaction of c-myc-transfected B-lymphoblastoid cell lines with resting and interleukin-1-activated endothelial cells. The transfected cell lines expressed lower levels of leukocyte function-associated antigen-1 compared with control non-transfected lines, while no reduction of expression of other surface structures, including the β1 integrin very late antigen-4, was observed. The transfected cell lines adhered to resting or activated endothelial cells less than control cells. Anti-CD18 monoclonal antibody inhibited binding of control cell lines but had a modest or no effect on adhesion of transfected cell lines. Anti-very late antigen-4 monoclonal antibody effectively inhibited binding of both transfected and control cell lines; this was more pronounced in the presence of anti-CD18, suggesting a cooperative interaction between these adhesion pathways. Transfected cell lines also had an impaired ability to penetrate endothelial cell monolayers in a transmigration assay. Our results indicate that activation of the c-myc oncogene in B-cells causes alterations in the adhesive interaction with endothelial cells. This may be relevant in the localization and malignant behavior of B-cell lymphomas carrying an activated c-myc oncogene.
Original language | English |
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Pages (from-to) | 29-32 |
Number of pages | 4 |
Journal | International Journal of Clinical & Laboratory Research |
Volume | 24 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 1994 |
Keywords
- Adhesion
- c-myc-Transfected B-lymphoblastoid cell lines
- Endothelial cells
- Leukocyte function-associated antigen-1
- Very late antigen-4
ASJC Scopus subject areas
- Clinical Biochemistry