Role of lipid peroxidation in the pathogenesis of myopic and senile cataract

T. Micelli-Ferrari, G. Vendemiale, I. Grattagliano, F. Boscia, L. Arnese, E. Altomare, L. Cardia

Research output: Contribution to journalArticle

Abstract

Aims/background - Increased production of free radicals, consumption of antioxidant, and oxidation of unsaturated lipids have been observed recently in cataractous lenses and active participation of the retina in human cataractogenesis has been proposed. To verify this hypothesis, the total (GSH) and oxidised (GSSG) glutathione concentrations were assayed in the lens and the malondialdehyde (MDA) levels assayed in the vitreous and in the lens of normal controls and patients with senile or myopic cataract. Methods-The study was conducted on 34 lenses (nucleus and epinucleus) (nine clear lenses, 14 lenses with idiopathic senile cataract, and 11 lenses affected by severe myopic cataract) and vitreous of 19 (seven non-myopic, seven myopic, and five control) subjects. Glutathione determination was performed following the method of Reed, while malondialdehyde was assayed using a modification of the method of Dahle. Results - Cataractous lenses showed a decreased content of GSH and increased concentration of GSSG compared with clear lenses. A higher oxidative consumption of GSH was found in myopic cataracts compared with senile ones. Also, increased levels of MDA were observed both in cataractous lenses and in the vitreous of myopic patients compared with the control and the senile ones. Conclusion - The observed alterations strongly suggest that retinal lipid peroxidation might play a key role in human cataractogenesis, especially in the myopic type.

Original languageEnglish
Pages (from-to)840-843
Number of pages4
JournalBritish Journal of Ophthalmology
Volume80
Issue number9
Publication statusPublished - 1996

Fingerprint

Cataract
Lipid Peroxidation
Lenses
Glutathione Disulfide
Malondialdehyde
Free Radicals
Glutathione
Retina
Antioxidants
Lipids

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Micelli-Ferrari, T., Vendemiale, G., Grattagliano, I., Boscia, F., Arnese, L., Altomare, E., & Cardia, L. (1996). Role of lipid peroxidation in the pathogenesis of myopic and senile cataract. British Journal of Ophthalmology, 80(9), 840-843.

Role of lipid peroxidation in the pathogenesis of myopic and senile cataract. / Micelli-Ferrari, T.; Vendemiale, G.; Grattagliano, I.; Boscia, F.; Arnese, L.; Altomare, E.; Cardia, L.

In: British Journal of Ophthalmology, Vol. 80, No. 9, 1996, p. 840-843.

Research output: Contribution to journalArticle

Micelli-Ferrari, T, Vendemiale, G, Grattagliano, I, Boscia, F, Arnese, L, Altomare, E & Cardia, L 1996, 'Role of lipid peroxidation in the pathogenesis of myopic and senile cataract', British Journal of Ophthalmology, vol. 80, no. 9, pp. 840-843.
Micelli-Ferrari T, Vendemiale G, Grattagliano I, Boscia F, Arnese L, Altomare E et al. Role of lipid peroxidation in the pathogenesis of myopic and senile cataract. British Journal of Ophthalmology. 1996;80(9):840-843.
Micelli-Ferrari, T. ; Vendemiale, G. ; Grattagliano, I. ; Boscia, F. ; Arnese, L. ; Altomare, E. ; Cardia, L. / Role of lipid peroxidation in the pathogenesis of myopic and senile cataract. In: British Journal of Ophthalmology. 1996 ; Vol. 80, No. 9. pp. 840-843.
@article{05b5719765bc4fd0bf752eae4b96eafa,
title = "Role of lipid peroxidation in the pathogenesis of myopic and senile cataract",
abstract = "Aims/background - Increased production of free radicals, consumption of antioxidant, and oxidation of unsaturated lipids have been observed recently in cataractous lenses and active participation of the retina in human cataractogenesis has been proposed. To verify this hypothesis, the total (GSH) and oxidised (GSSG) glutathione concentrations were assayed in the lens and the malondialdehyde (MDA) levels assayed in the vitreous and in the lens of normal controls and patients with senile or myopic cataract. Methods-The study was conducted on 34 lenses (nucleus and epinucleus) (nine clear lenses, 14 lenses with idiopathic senile cataract, and 11 lenses affected by severe myopic cataract) and vitreous of 19 (seven non-myopic, seven myopic, and five control) subjects. Glutathione determination was performed following the method of Reed, while malondialdehyde was assayed using a modification of the method of Dahle. Results - Cataractous lenses showed a decreased content of GSH and increased concentration of GSSG compared with clear lenses. A higher oxidative consumption of GSH was found in myopic cataracts compared with senile ones. Also, increased levels of MDA were observed both in cataractous lenses and in the vitreous of myopic patients compared with the control and the senile ones. Conclusion - The observed alterations strongly suggest that retinal lipid peroxidation might play a key role in human cataractogenesis, especially in the myopic type.",
author = "T. Micelli-Ferrari and G. Vendemiale and I. Grattagliano and F. Boscia and L. Arnese and E. Altomare and L. Cardia",
year = "1996",
language = "English",
volume = "80",
pages = "840--843",
journal = "British Journal of Ophthalmology",
issn = "0007-1161",
publisher = "BMJ Publishing Group",
number = "9",

}

TY - JOUR

T1 - Role of lipid peroxidation in the pathogenesis of myopic and senile cataract

AU - Micelli-Ferrari, T.

AU - Vendemiale, G.

AU - Grattagliano, I.

AU - Boscia, F.

AU - Arnese, L.

AU - Altomare, E.

AU - Cardia, L.

PY - 1996

Y1 - 1996

N2 - Aims/background - Increased production of free radicals, consumption of antioxidant, and oxidation of unsaturated lipids have been observed recently in cataractous lenses and active participation of the retina in human cataractogenesis has been proposed. To verify this hypothesis, the total (GSH) and oxidised (GSSG) glutathione concentrations were assayed in the lens and the malondialdehyde (MDA) levels assayed in the vitreous and in the lens of normal controls and patients with senile or myopic cataract. Methods-The study was conducted on 34 lenses (nucleus and epinucleus) (nine clear lenses, 14 lenses with idiopathic senile cataract, and 11 lenses affected by severe myopic cataract) and vitreous of 19 (seven non-myopic, seven myopic, and five control) subjects. Glutathione determination was performed following the method of Reed, while malondialdehyde was assayed using a modification of the method of Dahle. Results - Cataractous lenses showed a decreased content of GSH and increased concentration of GSSG compared with clear lenses. A higher oxidative consumption of GSH was found in myopic cataracts compared with senile ones. Also, increased levels of MDA were observed both in cataractous lenses and in the vitreous of myopic patients compared with the control and the senile ones. Conclusion - The observed alterations strongly suggest that retinal lipid peroxidation might play a key role in human cataractogenesis, especially in the myopic type.

AB - Aims/background - Increased production of free radicals, consumption of antioxidant, and oxidation of unsaturated lipids have been observed recently in cataractous lenses and active participation of the retina in human cataractogenesis has been proposed. To verify this hypothesis, the total (GSH) and oxidised (GSSG) glutathione concentrations were assayed in the lens and the malondialdehyde (MDA) levels assayed in the vitreous and in the lens of normal controls and patients with senile or myopic cataract. Methods-The study was conducted on 34 lenses (nucleus and epinucleus) (nine clear lenses, 14 lenses with idiopathic senile cataract, and 11 lenses affected by severe myopic cataract) and vitreous of 19 (seven non-myopic, seven myopic, and five control) subjects. Glutathione determination was performed following the method of Reed, while malondialdehyde was assayed using a modification of the method of Dahle. Results - Cataractous lenses showed a decreased content of GSH and increased concentration of GSSG compared with clear lenses. A higher oxidative consumption of GSH was found in myopic cataracts compared with senile ones. Also, increased levels of MDA were observed both in cataractous lenses and in the vitreous of myopic patients compared with the control and the senile ones. Conclusion - The observed alterations strongly suggest that retinal lipid peroxidation might play a key role in human cataractogenesis, especially in the myopic type.

UR - http://www.scopus.com/inward/record.url?scp=0029908782&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029908782&partnerID=8YFLogxK

M3 - Article

C2 - 8942384

AN - SCOPUS:0029908782

VL - 80

SP - 840

EP - 843

JO - British Journal of Ophthalmology

JF - British Journal of Ophthalmology

SN - 0007-1161

IS - 9

ER -