Role of Major Endocannabinoid-Binding Receptors during Mouse Oocyte Maturation

Sandra Cecconi, Gianna Rossi, Sergio Oddi, Valentina Di Nisio, Mauro Maccarrone

Research output: Contribution to journalArticle

Abstract

Endocannabinoids are key-players of female fertility and potential biomarkers of reproductive dysfunctions. Here, we investigated localization and expression of cannabinoid receptor type-1 and -2 (CB1R and CB2R), G-protein coupled receptor 55 (GPR55), and transient receptor potential vanilloid type 1 channel (TRPV1) in mouse oocytes collected at different stages of in vivo meiotic maturation (germinal vesicle, GV; metaphase I, MI; metaphase II, MII) through qPCR, confocal imaging, and western blot. Despite the significant decrease in CB1R, CB2R, and GPR55 mRNAs occurring from GV to MII, CB2R and GPR55 protein contents increased during the same period. At GV, only CB1R was localized in oolemma, but it completely disappeared at MI. TRPV1 was always undetectable. When oocytes were in vitro matured with CB1R and CB2R but not GPR55 antagonists, a significant delay of GV breakdown occurred, sustained by elevated intraoocyte cAMP concentration. Although CBRs antagonists did not affect polar body I emission or chromosome alignment, GPR55 antagonist impaired in ~75% of oocytes the formation of normal-sized MI and MII spindles. These findings open a new avenue to interrogate oocyte pathophysiology and offer potentially new targets for the therapy of reproductive alterations.

Original languageEnglish
JournalInternational Journal of Molecular Sciences
Volume20
Issue number12
DOIs
Publication statusPublished - Jun 12 2019

Fingerprint

gametocytes
Endocannabinoids
G-Protein-Coupled Receptors
Oocytes
mice
Proteins
proteins
Metaphase
Chromosomes, Human, 21-22 and Y
Polar Bodies
Cannabinoid Receptors
Biomarkers
Chromosomes
Fertility
fertility
Western Blotting
spindles
biomarkers
chromosomes
Imaging techniques

Keywords

  • endocannabinoids
  • meiosis
  • oocyte
  • receptors
  • signal transduction

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Role of Major Endocannabinoid-Binding Receptors during Mouse Oocyte Maturation. / Cecconi, Sandra; Rossi, Gianna; Oddi, Sergio; Di Nisio, Valentina; Maccarrone, Mauro.

In: International Journal of Molecular Sciences, Vol. 20, No. 12, 12.06.2019.

Research output: Contribution to journalArticle

@article{f7814e4ed53e40d78d52bb216cfbfcfb,
title = "Role of Major Endocannabinoid-Binding Receptors during Mouse Oocyte Maturation",
abstract = "Endocannabinoids are key-players of female fertility and potential biomarkers of reproductive dysfunctions. Here, we investigated localization and expression of cannabinoid receptor type-1 and -2 (CB1R and CB2R), G-protein coupled receptor 55 (GPR55), and transient receptor potential vanilloid type 1 channel (TRPV1) in mouse oocytes collected at different stages of in vivo meiotic maturation (germinal vesicle, GV; metaphase I, MI; metaphase II, MII) through qPCR, confocal imaging, and western blot. Despite the significant decrease in CB1R, CB2R, and GPR55 mRNAs occurring from GV to MII, CB2R and GPR55 protein contents increased during the same period. At GV, only CB1R was localized in oolemma, but it completely disappeared at MI. TRPV1 was always undetectable. When oocytes were in vitro matured with CB1R and CB2R but not GPR55 antagonists, a significant delay of GV breakdown occurred, sustained by elevated intraoocyte cAMP concentration. Although CBRs antagonists did not affect polar body I emission or chromosome alignment, GPR55 antagonist impaired in ~75{\%} of oocytes the formation of normal-sized MI and MII spindles. These findings open a new avenue to interrogate oocyte pathophysiology and offer potentially new targets for the therapy of reproductive alterations.",
keywords = "endocannabinoids, meiosis, oocyte, receptors, signal transduction",
author = "Sandra Cecconi and Gianna Rossi and Sergio Oddi and {Di Nisio}, Valentina and Mauro Maccarrone",
year = "2019",
month = "6",
day = "12",
doi = "10.3390/ijms20122866",
language = "English",
volume = "20",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "MDPI AG",
number = "12",

}

TY - JOUR

T1 - Role of Major Endocannabinoid-Binding Receptors during Mouse Oocyte Maturation

AU - Cecconi, Sandra

AU - Rossi, Gianna

AU - Oddi, Sergio

AU - Di Nisio, Valentina

AU - Maccarrone, Mauro

PY - 2019/6/12

Y1 - 2019/6/12

N2 - Endocannabinoids are key-players of female fertility and potential biomarkers of reproductive dysfunctions. Here, we investigated localization and expression of cannabinoid receptor type-1 and -2 (CB1R and CB2R), G-protein coupled receptor 55 (GPR55), and transient receptor potential vanilloid type 1 channel (TRPV1) in mouse oocytes collected at different stages of in vivo meiotic maturation (germinal vesicle, GV; metaphase I, MI; metaphase II, MII) through qPCR, confocal imaging, and western blot. Despite the significant decrease in CB1R, CB2R, and GPR55 mRNAs occurring from GV to MII, CB2R and GPR55 protein contents increased during the same period. At GV, only CB1R was localized in oolemma, but it completely disappeared at MI. TRPV1 was always undetectable. When oocytes were in vitro matured with CB1R and CB2R but not GPR55 antagonists, a significant delay of GV breakdown occurred, sustained by elevated intraoocyte cAMP concentration. Although CBRs antagonists did not affect polar body I emission or chromosome alignment, GPR55 antagonist impaired in ~75% of oocytes the formation of normal-sized MI and MII spindles. These findings open a new avenue to interrogate oocyte pathophysiology and offer potentially new targets for the therapy of reproductive alterations.

AB - Endocannabinoids are key-players of female fertility and potential biomarkers of reproductive dysfunctions. Here, we investigated localization and expression of cannabinoid receptor type-1 and -2 (CB1R and CB2R), G-protein coupled receptor 55 (GPR55), and transient receptor potential vanilloid type 1 channel (TRPV1) in mouse oocytes collected at different stages of in vivo meiotic maturation (germinal vesicle, GV; metaphase I, MI; metaphase II, MII) through qPCR, confocal imaging, and western blot. Despite the significant decrease in CB1R, CB2R, and GPR55 mRNAs occurring from GV to MII, CB2R and GPR55 protein contents increased during the same period. At GV, only CB1R was localized in oolemma, but it completely disappeared at MI. TRPV1 was always undetectable. When oocytes were in vitro matured with CB1R and CB2R but not GPR55 antagonists, a significant delay of GV breakdown occurred, sustained by elevated intraoocyte cAMP concentration. Although CBRs antagonists did not affect polar body I emission or chromosome alignment, GPR55 antagonist impaired in ~75% of oocytes the formation of normal-sized MI and MII spindles. These findings open a new avenue to interrogate oocyte pathophysiology and offer potentially new targets for the therapy of reproductive alterations.

KW - endocannabinoids

KW - meiosis

KW - oocyte

KW - receptors

KW - signal transduction

UR - http://www.scopus.com/inward/record.url?scp=85068476308&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068476308&partnerID=8YFLogxK

U2 - 10.3390/ijms20122866

DO - 10.3390/ijms20122866

M3 - Article

VL - 20

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 12

ER -