Role of microRNAs in host defense against Echinococcus granulosus infection: a preliminary assessment

Mara Mariconti, Ambra Vola, Tommaso Manciulli, Francesca Genco, Raffaella Lissandrin, Valeria Meroni, Mara Rosenzvit, Francesca Tamarozzi, Enrico Brunetti

Research output: Contribution to journalArticle

Abstract

Cystic echinococcosis (CE) is a neglected helminthic zoonosis caused by the larval stage of the tapeworm Echinococcus granulosus s.l. MicroRNAs (miRNAs) are regulators of gene expression that have been linked with the pathogenesis of several human diseases, but little exists in the available literature about miRNAs in CE. Here, we investigate the expression profiles of 84 microRNAs relevant to the function of lymphocytes and other immune cells during CE infection in the peripheral blood of patients with cysts in active and inactive stages. We applied the microRNA PCR array technology to blood samples from 20 patients with a single hepatic CE cyst in either the active (CE3b) or inactive (CE4–CE5) stage. Our results show a significant upregulation of eight miRNAs (let-7g-5p, let-7a-5p, miR- 26a-5p, miR- 26b-5p, miR- 195-5p, miR- 16-5p, miR- 30c-5p, and miR- 223-3p) in patients with active cysts compared to those with inactive cysts. The high expression of these miRNAs in patients with active cysts suggests their role in a specific host immune response against the infection. Further work in this direction may help shed light on the pathogenesis of human CE.

Original languageEnglish
JournalImmunologic Research
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Echinococcus granulosus
MicroRNAs
Echinococcosis
Cysts
Infection
Hepatic Echinococcosis
Cestoda
Zoonoses
Cerebral Palsy
Regulator Genes
Up-Regulation
Lymphocytes
Technology
Gene Expression
Polymerase Chain Reaction

Keywords

  • Cystic echinococcosis
  • Echinococcus granulosus
  • Hydatidosis
  • MicroRNA
  • Neglected disease
  • Zoonosis

ASJC Scopus subject areas

  • Immunology

Cite this

Role of microRNAs in host defense against Echinococcus granulosus infection : a preliminary assessment. / Mariconti, Mara; Vola, Ambra; Manciulli, Tommaso; Genco, Francesca; Lissandrin, Raffaella; Meroni, Valeria; Rosenzvit, Mara; Tamarozzi, Francesca; Brunetti, Enrico.

In: Immunologic Research, 01.01.2018.

Research output: Contribution to journalArticle

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AU - Genco, Francesca

AU - Lissandrin, Raffaella

AU - Meroni, Valeria

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AU - Tamarozzi, Francesca

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AB - Cystic echinococcosis (CE) is a neglected helminthic zoonosis caused by the larval stage of the tapeworm Echinococcus granulosus s.l. MicroRNAs (miRNAs) are regulators of gene expression that have been linked with the pathogenesis of several human diseases, but little exists in the available literature about miRNAs in CE. Here, we investigate the expression profiles of 84 microRNAs relevant to the function of lymphocytes and other immune cells during CE infection in the peripheral blood of patients with cysts in active and inactive stages. We applied the microRNA PCR array technology to blood samples from 20 patients with a single hepatic CE cyst in either the active (CE3b) or inactive (CE4–CE5) stage. Our results show a significant upregulation of eight miRNAs (let-7g-5p, let-7a-5p, miR- 26a-5p, miR- 26b-5p, miR- 195-5p, miR- 16-5p, miR- 30c-5p, and miR- 223-3p) in patients with active cysts compared to those with inactive cysts. The high expression of these miRNAs in patients with active cysts suggests their role in a specific host immune response against the infection. Further work in this direction may help shed light on the pathogenesis of human CE.

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