Role of miR-200c in Myogenic Differentiation Impairment via p66Shc: Implication in Skeletal Muscle Regeneration of Dystrophic mdx Mice

Marco D'Agostino, Alessio Torcinaro, Luca Madaro, Lorenza Marchetti, Sara Sileno, Sara Beji, Chiara Salis, Daisy Proietti, Giulia Imeneo, Maurizio C Capogrossi, Francesca De Santa, Alessandra Magenta

Research output: Contribution to journalArticle

Abstract

Duchenne muscular dystrophy (DMD) is a genetic disease associated with mutations of Dystrophin gene that regulate myofiber integrity and muscle degeneration, characterized by oxidative stress increase. We previously published that reactive oxygen species (ROS) induce miR-200c that is responsible for apoptosis and senescence. Moreover, we demonstrated that miR-200c increases ROS production and phosphorylates p66Shc in Ser-36. p66Shc plays an important role in muscle differentiation; we previously showed that p66Shc-/- muscle satellite cells display lower oxidative stress levels and higher proliferation rate and differentiated faster than wild-type (wt) cells. Moreover, myogenic conversion, induced by MyoD overexpression, is more efficient in p66Shc-/- fibroblasts compared to wt cells. Herein, we report that miR-200c overexpression in cultured myoblasts impairs skeletal muscle differentiation. Further, its overexpression in differentiated myotubes decreases differentiation indexes. Moreover, anti-miR-200c treatment ameliorates myogenic differentiation. In keeping, we found that miR-200c and p66Shc Ser-36 phosphorylation increase in mdx muscles. In conclusion, miR-200c inhibits muscle differentiation, whereas its inhibition ameliorates differentiation and its expression levels are increased in mdx mice and in differentiated human myoblasts of DMD. Therefore, miR-200c might be responsible for muscle wasting and myotube loss, most probably via a p66Shc-dependent mechanism in a pathological disease such as DMD.

Original languageEnglish
Pages (from-to)4814696
JournalOxidative Medicine and Cellular Longevity
Volume2018
DOIs
Publication statusPublished - 2018

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    D'Agostino, M., Torcinaro, A., Madaro, L., Marchetti, L., Sileno, S., Beji, S., Salis, C., Proietti, D., Imeneo, G., C Capogrossi, M., De Santa, F., & Magenta, A. (2018). Role of miR-200c in Myogenic Differentiation Impairment via p66Shc: Implication in Skeletal Muscle Regeneration of Dystrophic mdx Mice. Oxidative Medicine and Cellular Longevity, 2018, 4814696. https://doi.org/10.1155/2018/4814696