Role of mitochondria and reactive oxygen species in dendritic cell differentiation and functions

Annalisa Del Prete, Patrizia Zaccagnino, Marco Di Paola, Maddalena Saltarella, Constanza Oliveros Celis, Beatrice Nico, Giuseppe Santoro, Michele Lorusso

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Dendritic cells (DC) are potent antigen-presenting cells capable of inducing T and B responses and immune tolerance. We have characterized some aspects of energy metabolism accompanying the differentiation process of human monocytes into DC. Compared to precursor monocytes, DC exhibited a much larger number of mitochondria and consistently (i) a higher endogenous respiratory activity and (ii) a more than sixfold increase in ATP content and an even larger increase in the activity of the mitochondrial marker enzyme citrate synthase. The presence in the culture medium of rotenone, an inhibitor of the respiratory chain Complex I, prevented the increase in mitochondrial number and ATP level, without affecting cell viability. Rotenone inhibited DC differentiation, as revealed by the observation that the expression of CD1a, which is a specific surface marker of DC differentiation, was strongly reduced. Cells cultured in the presence of rotenone displayed a lower content of growth factor-induced, mitochondrially generated, hydrogen peroxide. A similar drop in ROS was observed upon addition of catalase, which caused functional effects similar to those produced by rotenone treatment. These results suggest that ROS play a crucial role in DC differentiation and that mitochondria are an important source of ROS in this process.

Original languageEnglish
Pages (from-to)1443-1451
Number of pages9
JournalFree Radical Biology and Medicine
Volume44
Issue number7
DOIs
Publication statusPublished - Apr 1 2008

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Mitochondria
Dendritic Cells
Rotenone
Cell Differentiation
Reactive Oxygen Species
Monocytes
Adenosine Triphosphate
Electron Transport Complex I
Citrate (si)-Synthase
Immune Tolerance
Antigen-Presenting Cells
Electron Transport
Catalase
Hydrogen Peroxide
Energy Metabolism
Culture Media
Cultured Cells
Cell Survival
Intercellular Signaling Peptides and Proteins
Cells

Keywords

  • Dendritic cells
  • Free radicals
  • Mitochondria
  • Oxidative phosphorylation
  • Reactive oxygen species

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Role of mitochondria and reactive oxygen species in dendritic cell differentiation and functions. / Del Prete, Annalisa; Zaccagnino, Patrizia; Di Paola, Marco; Saltarella, Maddalena; Oliveros Celis, Constanza; Nico, Beatrice; Santoro, Giuseppe; Lorusso, Michele.

In: Free Radical Biology and Medicine, Vol. 44, No. 7, 01.04.2008, p. 1443-1451.

Research output: Contribution to journalArticle

Del Prete, A, Zaccagnino, P, Di Paola, M, Saltarella, M, Oliveros Celis, C, Nico, B, Santoro, G & Lorusso, M 2008, 'Role of mitochondria and reactive oxygen species in dendritic cell differentiation and functions', Free Radical Biology and Medicine, vol. 44, no. 7, pp. 1443-1451. https://doi.org/10.1016/j.freeradbiomed.2007.12.037
Del Prete, Annalisa ; Zaccagnino, Patrizia ; Di Paola, Marco ; Saltarella, Maddalena ; Oliveros Celis, Constanza ; Nico, Beatrice ; Santoro, Giuseppe ; Lorusso, Michele. / Role of mitochondria and reactive oxygen species in dendritic cell differentiation and functions. In: Free Radical Biology and Medicine. 2008 ; Vol. 44, No. 7. pp. 1443-1451.
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