Role of mucosal high-risk human papillomavirus types in head and neck cancers in Romania

Ramona Gabriela Ursu; Mihai Danciu; Irene Alexandra Spiridon; Ruediger Ridder; Susanne Rehm; Fausto Maffini; Sandrine McKay-Chopin; Christine Carreira; Eric Lucas; Victor Vlad Costan; Eugenia Popescu; Bogdan Cobzeanu; Nicolae Ghetu; Luminita Smaranda Iancu; Massimo Tommasino; Michael Pawlita; Dana Holzinger; Tarik Gheit

doi:10.1371/journal.pone.0199663

Role of mucosal high-risk human papillomavirus types in head and neck cancers in Romania

Ramona Gabriela Ursu, Mihai Danciu, Irene Alexandra Spiridon, Ruediger Ridder, Susanne Rehm, Fausto Maffini, Sandrine McKay-Chopin, Christine Carreira, Eric Lucas, Victor Vlad Costan, Eugenia Popescu, Bogdan Cobzeanu, Nicolae Ghetu, Luminita Smaranda Iancu, Massimo Tommasino, Michael Pawlita, Dana Holzinger, Tarik Gheit

Istituto Europeo di Oncologia

Research output: Contribution to journal › Article

Abstract

Background Limited information is available about the involvement of human papillomavirus (HPV) in head and neck squamous cell carcinomas (HNSCCs) in Romanian patients. Objective To evaluate the HPV-attributable fraction in HNSCCs collected in Northeastern Romania. Materials and methods In total, 189 formalin-fixed paraffin-embedded tissue samples (99 oral cavity tumors, 28 oropharynx, 48 pharynx, and 14 larynx/hypopharynx) were analyzed for HPV DNA and RNA using Luminex-based assays, and for overexpression of p16^INK4a (p16) by immunohistochemistry. Results Of the 189 cases, 23 (12.2%) were HPV DNA-positive, comprising half of the oropharyngeal cases (14/28, 50.0%) and 9/161 (5.6%) of the non-oropharyngeal cases. HPV16 was the most prevalent HPV type (20/23, 86.9%), followed by HPV18 (5/23, 21.7%) and HPV39 (1/23, 4.3%). Only two (2/189, 1.1%) HNSCC cases were HPV-driven, i.e. positive for both HPV DNA and RNA. Conclusion A very small subset of HNSCC cases within this cohort from Northeastern Romania appeared to be HPV-driven.

Original language	English
Article number	e0199663
Journal	PLoS One
Volume	13
Issue number	6
DOIs	https://doi.org/10.1371/journal.pone.0199663
Publication status	Published - Jun 1 2018

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

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Ursu, R. G., Danciu, M., Spiridon, I. A., Ridder, R., Rehm, S., Maffini, F., McKay-Chopin, S., Carreira, C., Lucas, E., Costan, V. V., Popescu, E., Cobzeanu, B., Ghetu, N., Iancu, L. S., Tommasino, M., Pawlita, M., Holzinger, D., & Gheit, T. (2018). Role of mucosal high-risk human papillomavirus types in head and neck cancers in Romania. PLoS One, 13(6), [e0199663]. https://doi.org/10.1371/journal.pone.0199663

Role of mucosal high-risk human papillomavirus types in head and neck cancers in Romania. / Ursu, Ramona Gabriela; Danciu, Mihai; Spiridon, Irene Alexandra; Ridder, Ruediger; Rehm, Susanne; Maffini, Fausto; McKay-Chopin, Sandrine; Carreira, Christine; Lucas, Eric; Costan, Victor Vlad; Popescu, Eugenia; Cobzeanu, Bogdan; Ghetu, Nicolae; Iancu, Luminita Smaranda; Tommasino, Massimo; Pawlita, Michael; Holzinger, Dana; Gheit, Tarik.

In: PLoS One, Vol. 13, No. 6, e0199663, 01.06.2018.

Research output: Contribution to journal › Article

Ursu, RG, Danciu, M, Spiridon, IA, Ridder, R, Rehm, S, Maffini, F, McKay-Chopin, S, Carreira, C, Lucas, E, Costan, VV, Popescu, E, Cobzeanu, B, Ghetu, N, Iancu, LS, Tommasino, M, Pawlita, M, Holzinger, D & Gheit, T 2018, 'Role of mucosal high-risk human papillomavirus types in head and neck cancers in Romania', PLoS One, vol. 13, no. 6, e0199663. https://doi.org/10.1371/journal.pone.0199663

Ursu, Ramona Gabriela ; Danciu, Mihai ; Spiridon, Irene Alexandra ; Ridder, Ruediger ; Rehm, Susanne ; Maffini, Fausto ; McKay-Chopin, Sandrine ; Carreira, Christine ; Lucas, Eric ; Costan, Victor Vlad ; Popescu, Eugenia ; Cobzeanu, Bogdan ; Ghetu, Nicolae ; Iancu, Luminita Smaranda ; Tommasino, Massimo ; Pawlita, Michael ; Holzinger, Dana ; Gheit, Tarik. / Role of mucosal high-risk human papillomavirus types in head and neck cancers in Romania. In: PLoS One. 2018 ; Vol. 13, No. 6.

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abstract = "Background Limited information is available about the involvement of human papillomavirus (HPV) in head and neck squamous cell carcinomas (HNSCCs) in Romanian patients. Objective To evaluate the HPV-attributable fraction in HNSCCs collected in Northeastern Romania. Materials and methods In total, 189 formalin-fixed paraffin-embedded tissue samples (99 oral cavity tumors, 28 oropharynx, 48 pharynx, and 14 larynx/hypopharynx) were analyzed for HPV DNA and RNA using Luminex-based assays, and for overexpression of p16INK4a (p16) by immunohistochemistry. Results Of the 189 cases, 23 (12.2%) were HPV DNA-positive, comprising half of the oropharyngeal cases (14/28, 50.0%) and 9/161 (5.6%) of the non-oropharyngeal cases. HPV16 was the most prevalent HPV type (20/23, 86.9%), followed by HPV18 (5/23, 21.7%) and HPV39 (1/23, 4.3%). Only two (2/189, 1.1%) HNSCC cases were HPV-driven, i.e. positive for both HPV DNA and RNA. Conclusion A very small subset of HNSCC cases within this cohort from Northeastern Romania appeared to be HPV-driven.",

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AU - Rehm, Susanne

AU - Maffini, Fausto

AU - McKay-Chopin, Sandrine

AU - Carreira, Christine

AU - Lucas, Eric

AU - Costan, Victor Vlad

AU - Popescu, Eugenia

AU - Cobzeanu, Bogdan

AU - Ghetu, Nicolae

AU - Iancu, Luminita Smaranda

AU - Tommasino, Massimo

AU - Pawlita, Michael

AU - Holzinger, Dana

AU - Gheit, Tarik

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N2 - Background Limited information is available about the involvement of human papillomavirus (HPV) in head and neck squamous cell carcinomas (HNSCCs) in Romanian patients. Objective To evaluate the HPV-attributable fraction in HNSCCs collected in Northeastern Romania. Materials and methods In total, 189 formalin-fixed paraffin-embedded tissue samples (99 oral cavity tumors, 28 oropharynx, 48 pharynx, and 14 larynx/hypopharynx) were analyzed for HPV DNA and RNA using Luminex-based assays, and for overexpression of p16INK4a (p16) by immunohistochemistry. Results Of the 189 cases, 23 (12.2%) were HPV DNA-positive, comprising half of the oropharyngeal cases (14/28, 50.0%) and 9/161 (5.6%) of the non-oropharyngeal cases. HPV16 was the most prevalent HPV type (20/23, 86.9%), followed by HPV18 (5/23, 21.7%) and HPV39 (1/23, 4.3%). Only two (2/189, 1.1%) HNSCC cases were HPV-driven, i.e. positive for both HPV DNA and RNA. Conclusion A very small subset of HNSCC cases within this cohort from Northeastern Romania appeared to be HPV-driven.

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