Role of mucosal high-risk human papillomavirus types in head and neck cancers in Romania

Ramona Gabriela Ursu, Mihai Danciu, Irene Alexandra Spiridon, Ruediger Ridder, Susanne Rehm, Fausto Maffini, Sandrine McKay-Chopin, Christine Carreira, Eric Lucas, Victor Vlad Costan, Eugenia Popescu, Bogdan Cobzeanu, Nicolae Ghetu, Luminita Smaranda Iancu, Massimo Tommasino, Michael Pawlita, Dana Holzinger, Tarik Gheit

Research output: Contribution to journalArticlepeer-review

Abstract

Background Limited information is available about the involvement of human papillomavirus (HPV) in head and neck squamous cell carcinomas (HNSCCs) in Romanian patients. Objective To evaluate the HPV-attributable fraction in HNSCCs collected in Northeastern Romania. Materials and methods In total, 189 formalin-fixed paraffin-embedded tissue samples (99 oral cavity tumors, 28 oropharynx, 48 pharynx, and 14 larynx/hypopharynx) were analyzed for HPV DNA and RNA using Luminex-based assays, and for overexpression of p16INK4a (p16) by immunohistochemistry. Results Of the 189 cases, 23 (12.2%) were HPV DNA-positive, comprising half of the oropharyngeal cases (14/28, 50.0%) and 9/161 (5.6%) of the non-oropharyngeal cases. HPV16 was the most prevalent HPV type (20/23, 86.9%), followed by HPV18 (5/23, 21.7%) and HPV39 (1/23, 4.3%). Only two (2/189, 1.1%) HNSCC cases were HPV-driven, i.e. positive for both HPV DNA and RNA. Conclusion A very small subset of HNSCC cases within this cohort from Northeastern Romania appeared to be HPV-driven.

Original languageEnglish
Article numbere0199663
JournalPLoS One
Volume13
Issue number6
DOIs
Publication statusPublished - Jun 1 2018

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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