Role of mucosal high-risk human papillomavirus types in head and neck cancers in Romania

Ramona Gabriela Ursu, Mihai Danciu, Irene Alexandra Spiridon, Ruediger Ridder, Susanne Rehm, Fausto Maffini, Sandrine McKay-Chopin, Christine Carreira, Eric Lucas, Victor Vlad Costan, Eugenia Popescu, Bogdan Cobzeanu, Nicolae Ghetu, Luminita Smaranda Iancu, Massimo Tommasino, Michael Pawlita, Dana Holzinger, Tarik Gheit

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background Limited information is available about the involvement of human papillomavirus (HPV) in head and neck squamous cell carcinomas (HNSCCs) in Romanian patients. Objective To evaluate the HPV-attributable fraction in HNSCCs collected in Northeastern Romania. Materials and methods In total, 189 formalin-fixed paraffin-embedded tissue samples (99 oral cavity tumors, 28 oropharynx, 48 pharynx, and 14 larynx/hypopharynx) were analyzed for HPV DNA and RNA using Luminex-based assays, and for overexpression of p16INK4a (p16) by immunohistochemistry. Results Of the 189 cases, 23 (12.2%) were HPV DNA-positive, comprising half of the oropharyngeal cases (14/28, 50.0%) and 9/161 (5.6%) of the non-oropharyngeal cases. HPV16 was the most prevalent HPV type (20/23, 86.9%), followed by HPV18 (5/23, 21.7%) and HPV39 (1/23, 4.3%). Only two (2/189, 1.1%) HNSCC cases were HPV-driven, i.e. positive for both HPV DNA and RNA. Conclusion A very small subset of HNSCC cases within this cohort from Northeastern Romania appeared to be HPV-driven.

Original languageEnglish
Article numbere0199663
JournalPLoS One
Volume13
Issue number6
DOIs
Publication statusPublished - Jun 1 2018

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Romania
Papillomaviridae
Head and Neck Neoplasms
squamous cell carcinoma
neck
DNA
RNA
Paraffin
Formaldehyde
Tumors
Assays
Tissue
Hypopharynx
Oropharynx
larynx
head and neck neoplasms
Epithelial Cells
pharynx
Larynx
Pharynx

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Ursu, R. G., Danciu, M., Spiridon, I. A., Ridder, R., Rehm, S., Maffini, F., ... Gheit, T. (2018). Role of mucosal high-risk human papillomavirus types in head and neck cancers in Romania. PLoS One, 13(6), [e0199663]. https://doi.org/10.1371/journal.pone.0199663

Role of mucosal high-risk human papillomavirus types in head and neck cancers in Romania. / Ursu, Ramona Gabriela; Danciu, Mihai; Spiridon, Irene Alexandra; Ridder, Ruediger; Rehm, Susanne; Maffini, Fausto; McKay-Chopin, Sandrine; Carreira, Christine; Lucas, Eric; Costan, Victor Vlad; Popescu, Eugenia; Cobzeanu, Bogdan; Ghetu, Nicolae; Iancu, Luminita Smaranda; Tommasino, Massimo; Pawlita, Michael; Holzinger, Dana; Gheit, Tarik.

In: PLoS One, Vol. 13, No. 6, e0199663, 01.06.2018.

Research output: Contribution to journalArticle

Ursu, RG, Danciu, M, Spiridon, IA, Ridder, R, Rehm, S, Maffini, F, McKay-Chopin, S, Carreira, C, Lucas, E, Costan, VV, Popescu, E, Cobzeanu, B, Ghetu, N, Iancu, LS, Tommasino, M, Pawlita, M, Holzinger, D & Gheit, T 2018, 'Role of mucosal high-risk human papillomavirus types in head and neck cancers in Romania', PLoS One, vol. 13, no. 6, e0199663. https://doi.org/10.1371/journal.pone.0199663
Ursu, Ramona Gabriela ; Danciu, Mihai ; Spiridon, Irene Alexandra ; Ridder, Ruediger ; Rehm, Susanne ; Maffini, Fausto ; McKay-Chopin, Sandrine ; Carreira, Christine ; Lucas, Eric ; Costan, Victor Vlad ; Popescu, Eugenia ; Cobzeanu, Bogdan ; Ghetu, Nicolae ; Iancu, Luminita Smaranda ; Tommasino, Massimo ; Pawlita, Michael ; Holzinger, Dana ; Gheit, Tarik. / Role of mucosal high-risk human papillomavirus types in head and neck cancers in Romania. In: PLoS One. 2018 ; Vol. 13, No. 6.
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abstract = "Background Limited information is available about the involvement of human papillomavirus (HPV) in head and neck squamous cell carcinomas (HNSCCs) in Romanian patients. Objective To evaluate the HPV-attributable fraction in HNSCCs collected in Northeastern Romania. Materials and methods In total, 189 formalin-fixed paraffin-embedded tissue samples (99 oral cavity tumors, 28 oropharynx, 48 pharynx, and 14 larynx/hypopharynx) were analyzed for HPV DNA and RNA using Luminex-based assays, and for overexpression of p16INK4a (p16) by immunohistochemistry. Results Of the 189 cases, 23 (12.2{\%}) were HPV DNA-positive, comprising half of the oropharyngeal cases (14/28, 50.0{\%}) and 9/161 (5.6{\%}) of the non-oropharyngeal cases. HPV16 was the most prevalent HPV type (20/23, 86.9{\%}), followed by HPV18 (5/23, 21.7{\%}) and HPV39 (1/23, 4.3{\%}). Only two (2/189, 1.1{\%}) HNSCC cases were HPV-driven, i.e. positive for both HPV DNA and RNA. Conclusion A very small subset of HNSCC cases within this cohort from Northeastern Romania appeared to be HPV-driven.",
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AU - Spiridon, Irene Alexandra

AU - Ridder, Ruediger

AU - Rehm, Susanne

AU - Maffini, Fausto

AU - McKay-Chopin, Sandrine

AU - Carreira, Christine

AU - Lucas, Eric

AU - Costan, Victor Vlad

AU - Popescu, Eugenia

AU - Cobzeanu, Bogdan

AU - Ghetu, Nicolae

AU - Iancu, Luminita Smaranda

AU - Tommasino, Massimo

AU - Pawlita, Michael

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AU - Gheit, Tarik

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N2 - Background Limited information is available about the involvement of human papillomavirus (HPV) in head and neck squamous cell carcinomas (HNSCCs) in Romanian patients. Objective To evaluate the HPV-attributable fraction in HNSCCs collected in Northeastern Romania. Materials and methods In total, 189 formalin-fixed paraffin-embedded tissue samples (99 oral cavity tumors, 28 oropharynx, 48 pharynx, and 14 larynx/hypopharynx) were analyzed for HPV DNA and RNA using Luminex-based assays, and for overexpression of p16INK4a (p16) by immunohistochemistry. Results Of the 189 cases, 23 (12.2%) were HPV DNA-positive, comprising half of the oropharyngeal cases (14/28, 50.0%) and 9/161 (5.6%) of the non-oropharyngeal cases. HPV16 was the most prevalent HPV type (20/23, 86.9%), followed by HPV18 (5/23, 21.7%) and HPV39 (1/23, 4.3%). Only two (2/189, 1.1%) HNSCC cases were HPV-driven, i.e. positive for both HPV DNA and RNA. Conclusion A very small subset of HNSCC cases within this cohort from Northeastern Romania appeared to be HPV-driven.

AB - Background Limited information is available about the involvement of human papillomavirus (HPV) in head and neck squamous cell carcinomas (HNSCCs) in Romanian patients. Objective To evaluate the HPV-attributable fraction in HNSCCs collected in Northeastern Romania. Materials and methods In total, 189 formalin-fixed paraffin-embedded tissue samples (99 oral cavity tumors, 28 oropharynx, 48 pharynx, and 14 larynx/hypopharynx) were analyzed for HPV DNA and RNA using Luminex-based assays, and for overexpression of p16INK4a (p16) by immunohistochemistry. Results Of the 189 cases, 23 (12.2%) were HPV DNA-positive, comprising half of the oropharyngeal cases (14/28, 50.0%) and 9/161 (5.6%) of the non-oropharyngeal cases. HPV16 was the most prevalent HPV type (20/23, 86.9%), followed by HPV18 (5/23, 21.7%) and HPV39 (1/23, 4.3%). Only two (2/189, 1.1%) HNSCC cases were HPV-driven, i.e. positive for both HPV DNA and RNA. Conclusion A very small subset of HNSCC cases within this cohort from Northeastern Romania appeared to be HPV-driven.

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