Role of pescadillo and upstream binding factor in the proliferation and differentiation of murine myeloid cells

Marco Prisco, Arianna Maiorana, Clara Guerzoni, George Calin, Bruno Calabretta, Renate Voit, Ingrid Grummt, Renato Baserga

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Pescadillo (PES1) and the upstream binding factor (UBF1) play a role in ribosome biogenesis, which regulates cell size, an important component of cell proliferation. We have investigated the effects of PES1 and UBF1 on the growth and differentiation of cell lines derived from 32D cells, an interleukin-3 (IL-3)-dependent murine myeloid cell line. Parental 32D cells and 32D IGF-IR cells (expressing increased levels of the type 1 insulin-like growth factor I [IGF-I] receptor [IGF-IR]) do not express insulin receptor substrate 1 (IRS-1) or IRS-2. 32D IGF-IR cells differentiate when the cells are shifted from IL-3 to IGF-I. Ectopic expression of IRS-1 inhibits differentiation and transforms 32D IGF-IR cells into a tumor-forming cell line. We found that PES1 and UBF1 increased cell size and/or altered the cell cycle distribution of 32D-derived cells but failed to make them IL-3 independent. PES1 and UBF1 also failed to inhibit the differentiation program initiated by the activation of the IGF-IR, which is blocked by IRS-1. 32D IGF-IR cells expressing PES1 or UBF1 differentiate into granulocytes like their parental cells. In contrast, PES1 and UBF1 can transform mouse embryo fibroblasts that have high levels of endogenous IRS-1 and are not prone to differentiation. Our results provide a model for one of the theories of myeloid leukemia, in which both a stimulus of proliferation and a block of differentiation are required for leukemia development.

Original languageEnglish
Pages (from-to)5421-5433
Number of pages13
JournalMolecular and Cellular Biology
Volume24
Issue number12
DOIs
Publication statusPublished - Jun 2004

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Myeloid Cells
IGF Type 1 Receptor
Insulin Receptor Substrate Proteins
Interleukin-3
Cell Size
transcription factor UBF
Cell Line
Myeloid Leukemia
Tumor Cell Line
Insulin-Like Growth Factor I
Ribosomes
Granulocytes
Cell Cycle
Leukemia
Embryonic Structures
Fibroblasts
Cell Proliferation
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Role of pescadillo and upstream binding factor in the proliferation and differentiation of murine myeloid cells. / Prisco, Marco; Maiorana, Arianna; Guerzoni, Clara; Calin, George; Calabretta, Bruno; Voit, Renate; Grummt, Ingrid; Baserga, Renato.

In: Molecular and Cellular Biology, Vol. 24, No. 12, 06.2004, p. 5421-5433.

Research output: Contribution to journalArticle

Prisco, M, Maiorana, A, Guerzoni, C, Calin, G, Calabretta, B, Voit, R, Grummt, I & Baserga, R 2004, 'Role of pescadillo and upstream binding factor in the proliferation and differentiation of murine myeloid cells', Molecular and Cellular Biology, vol. 24, no. 12, pp. 5421-5433. https://doi.org/10.1128/MCB.24.12.5421-5433.2004
Prisco, Marco ; Maiorana, Arianna ; Guerzoni, Clara ; Calin, George ; Calabretta, Bruno ; Voit, Renate ; Grummt, Ingrid ; Baserga, Renato. / Role of pescadillo and upstream binding factor in the proliferation and differentiation of murine myeloid cells. In: Molecular and Cellular Biology. 2004 ; Vol. 24, No. 12. pp. 5421-5433.
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