Role of phospholipids in the activation of the Ca2+-dependent neutral proteinase of human erythrocytes

S. Pontremoli, E. Melloni, B. Sparatore, F. Salamino, M. Michetti, O. Sacco, B. L. Horecker

Research output: Contribution to journalArticlepeer-review

Abstract

Activation of the Ca2+-dependent neutral proteinase of human erythrocytes in the presence of Ca2+ and a digestible substrate (Pontremoli, S., Sparatore, B., Melloni, E., Michetti, M. and Horecker, B.L. 1984, Biochem. Biophys. Res. Communs. 123, 331-337) is promoted by phospholipids such as phosphatidylcholine, phosphatidylinositol and phosphatidylserine. The presence of at least one unsaturated fatty acid chain is essential and metabolic derivatives such as dioleylglycerol, phosphorylserine and free fatty acids are ineffective. The most effective promoter was a freshly prepared mixture of phospholipids from human erythrocyte membranes. Activation involves conversion of the 80 kDa proenzyme (procalpain) subunit to the 75 kDa active proteinase and is irreversible. Phospholipids act by producing a large decrease in the concentration of Ca2+ required for the conversion of procalpain to active calpain.

Original languageEnglish
Pages (from-to)389-395
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume129
Issue number2
DOIs
Publication statusPublished - Jun 14 1985

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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