Role of prostaglandins in the early phases of experimental gastric carcinogenesis in the rat

A. Materia, G. Silecchia, E. Spaziani, P. Mariani, L. Scucchi, P. Mingazzini, C. Favalli, E. Garaci, N. Basso

Research output: Contribution to journalArticle

Abstract

The study was initiated to evaluate the effect of N-methyl-N-nitro-N-nitrosoguanidine (NG) on gastric intraluminal prostaglandin release during a 30-day treatment period and to investigate the effect of a stable prostaglandin E1 analogue (misoprostol) on NG-induced gastric mucosal damage during the same time period. Samples of gastric juice (1 h) were obtained from 40 male Sprague-Dawley rats with chronic gastric fistulas, in basal conditions and after 5, 15 and 30 days of continuous oral administration of NG (120 mg/l) or tap water. Aliquots of gastric juice were titrated with 0.1 M NaOH. Other aliquots were extracted with ethyl acetate and subjected to specific radioimmunoassay for prostaglandin E2. The severity of gastric mucosal lesions was evaluated in 60 rats after 5 days and 30 days of continuous oral administration of NG (120 mg/l) or NG plus misoprostol (200 μg/kg-1/day-1) or tap water, and a histological study was carried out. Administration of NG induced a significant decrease of gastric intraluminal prostaglandin E2 concentration at 15 and 30 days. Oral administration of misoprostol, at non-antisecretory doses, protected the rats against NG-induced gastric mucosal damage. Prostaglandins may be involved in the early phases of experimental gastric carcinogenesis.

Original languageEnglish
Pages (from-to)253-258
Number of pages6
JournalJournal of Cancer Research and Clinical Oncology
Volume115
Issue number3
DOIs
Publication statusPublished - Jun 1989

Keywords

  • Gastric experimental carcinogenesis
  • Gastric juice
  • N-Methyl-N-nitro-N-nitrosoguanidine
  • Prostaglandin analogues
  • Prostaglandins

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Materia, A., Silecchia, G., Spaziani, E., Mariani, P., Scucchi, L., Mingazzini, P., Favalli, C., Garaci, E., & Basso, N. (1989). Role of prostaglandins in the early phases of experimental gastric carcinogenesis in the rat. Journal of Cancer Research and Clinical Oncology, 115(3), 253-258. https://doi.org/10.1007/BF00391698