Role of skin surface lipids in UV-induced epidermal cell changes

M. Picardo, C. Zompetta, C. De Luca, M. Cirone, A. Faggioni, M. Nazzaro-Porro, S. Passi, G. Prota

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Ultraviolet irradiation is capable of affecting skin surface lipids, especially squalene and cholesterol, both in vitro and in vivo, with generation of active lipoperoxides. The photodecomposition of the skin lipid component was carefully evaluated by capillary gas-chromatography. The effects of UV-induced lipoperoxides on human keratinocytes in culture and on guinea pig ear slices were compared with those of synthetic lipoperoxides, i.e. cumene hydroperoxide and 13-hydroperoxylinoleate. A time- and dose-dependent effect on protein synthesis and mitotic activity was observed. In cell culture low concentrations (0.05-5 Μg/ml) of peroxidated squalene and synthetic lipoperoxides stimulated the incorporation of radiolabelled thymidine and phenylalanine, while higher concentrations (>10 Μg/ml), or longer periods of treatment, induced cellular damage. In guinea pig ear slices, the lipoperoxides (5-50 Μg/ml) increased aminoacid incorporation and the number of epidermal pigment cells; higher concentrations (>100 Μg/ml) caused a derangement of epidermal structure. The results suggest that UV irradiation of skin generates lipoperoxides from the surface lipids which, in vitro, are capable of producing a number of changes in epidermal cells.

Original languageEnglish
Pages (from-to)191-197
Number of pages7
JournalArchives of Dermatological Research
Volume283
Issue number3
DOIs
Publication statusPublished - May 1991

Fingerprint

Lipid Peroxides
Lipids
Skin
Squalene
Ear
Guinea Pigs
Phenylalanine
Keratinocytes
Gas Chromatography
Thymidine
Cell Culture Techniques
Cholesterol
Proteins

Keywords

  • Lipids
  • Lipoperoxidation
  • Skin surface
  • Squalene
  • UV radiation

ASJC Scopus subject areas

  • Dermatology

Cite this

Picardo, M., Zompetta, C., De Luca, C., Cirone, M., Faggioni, A., Nazzaro-Porro, M., ... Prota, G. (1991). Role of skin surface lipids in UV-induced epidermal cell changes. Archives of Dermatological Research, 283(3), 191-197. https://doi.org/10.1007/BF00372061

Role of skin surface lipids in UV-induced epidermal cell changes. / Picardo, M.; Zompetta, C.; De Luca, C.; Cirone, M.; Faggioni, A.; Nazzaro-Porro, M.; Passi, S.; Prota, G.

In: Archives of Dermatological Research, Vol. 283, No. 3, 05.1991, p. 191-197.

Research output: Contribution to journalArticle

Picardo, M, Zompetta, C, De Luca, C, Cirone, M, Faggioni, A, Nazzaro-Porro, M, Passi, S & Prota, G 1991, 'Role of skin surface lipids in UV-induced epidermal cell changes', Archives of Dermatological Research, vol. 283, no. 3, pp. 191-197. https://doi.org/10.1007/BF00372061
Picardo M, Zompetta C, De Luca C, Cirone M, Faggioni A, Nazzaro-Porro M et al. Role of skin surface lipids in UV-induced epidermal cell changes. Archives of Dermatological Research. 1991 May;283(3):191-197. https://doi.org/10.1007/BF00372061
Picardo, M. ; Zompetta, C. ; De Luca, C. ; Cirone, M. ; Faggioni, A. ; Nazzaro-Porro, M. ; Passi, S. ; Prota, G. / Role of skin surface lipids in UV-induced epidermal cell changes. In: Archives of Dermatological Research. 1991 ; Vol. 283, No. 3. pp. 191-197.
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