TY - JOUR
T1 - Role of the calpain-calpastatin system in the density-dependent growth arrest
AU - Stifanese, Roberto
AU - Averna, Monica
AU - De Tullio, Roberta
AU - Salamino, Franca
AU - Cantoni, Claudia
AU - Mingari, Maria Cristina
AU - Prato, Carola
AU - Pontremoli, Sandro
AU - Melloni, Edon
PY - 2008/11/15
Y1 - 2008/11/15
N2 - In dividing cells calpastatin diffuses from aggregates into cytosol, indicating the requirement for a tight regulation of calpain. Accordingly, the involvement of the calpain-calpastatin system in cell proliferation and in the density-dependent growth arrest was studied in JA3 cells stably transfected with a calpastatin form permanently localized in cytosol. In calpastatin overexpressing cells, cell cycle rate is 50% reduced, and cells enter the ungrowing, still fully reversible, stage at a 3-fold higher cell density. Furthermore, in cell density growth arrest phase, down regulation of α- and θ-PKC isoforms, as well as FAK and talin occurs. In calpastatin overexpressing cells, degradation of these calpain substrate proteins is prevented and delayed. Thus, calpain activity plays a crucial role in inducing the cell entry into a functional quiescent phase.
AB - In dividing cells calpastatin diffuses from aggregates into cytosol, indicating the requirement for a tight regulation of calpain. Accordingly, the involvement of the calpain-calpastatin system in cell proliferation and in the density-dependent growth arrest was studied in JA3 cells stably transfected with a calpastatin form permanently localized in cytosol. In calpastatin overexpressing cells, cell cycle rate is 50% reduced, and cells enter the ungrowing, still fully reversible, stage at a 3-fold higher cell density. Furthermore, in cell density growth arrest phase, down regulation of α- and θ-PKC isoforms, as well as FAK and talin occurs. In calpastatin overexpressing cells, degradation of these calpain substrate proteins is prevented and delayed. Thus, calpain activity plays a crucial role in inducing the cell entry into a functional quiescent phase.
KW - Calpain
KW - Calpastatin
KW - Cell growth arrest
KW - Cell proliferation
KW - Down regulation
KW - Focal adhesion kinase (FAK)
KW - Protein kinase C
KW - Talin
UR - http://www.scopus.com/inward/record.url?scp=54249145521&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=54249145521&partnerID=8YFLogxK
U2 - 10.1016/j.abb.2008.09.002
DO - 10.1016/j.abb.2008.09.002
M3 - Article
C2 - 18809371
AN - SCOPUS:54249145521
VL - 479
SP - 145
EP - 152
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
SN - 0003-9861
IS - 2
ER -