Role of the Na+-Ca2+ and Na+-H+ antiporters in prolactin release from anterior pituitary cells in primary culture

Gianfranco Di Renzo, Salvatore Amoroso, Antonella Bassi, Alessandro Fatatis, Mauro Cataldi, Anna Maria Colao, Gaetano Lombardi, Lucio Annunziato

Research output: Contribution to journalArticle

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Abstract

2′,4′-dimethylbenzamilamiloride (DMB), a somewhat selective inhibitor of the Na+-Ca2+ exchanger, in concentrations of 10, 30 and 100 μM did not produce any significant effect on baseline prolactin release from anterior pituitary cells in primary culture. When prolactin secretion was stimulated by the inhibitor of the Na+-K+-ATPase, ouabain, that activates the Na+-Ca2+ exchanger as a Ca2+-influx pathway, DMB was able to produce inhibition of prolactin secretion. 5-(N,N-hexamethylene) amiloride (HMA), another amiloride analog which specifically inhibits the Na+-H+ antiporter and has no inhibitory activity on the Na+-Ca2+ exchanger, at the concentrations of 0.1, 1 and 10 μM, did not affect basal prolactin release whereas it significantly reduced prolactin release stimulated by thyrotropin releasing hormone (TRH) (1 μM). These results suggest that the Na+-Ca2+ antiporter is involved in the process of prolactin release elicited by the inhibition of the Na+-K+-ATPase whereas the Na +-H+ antiporter is involved in the prolactin secretion elicited by TRH.

Original languageEnglish
Pages (from-to)11-15
Number of pages5
JournalEuropean Journal of Pharmacology
Volume294
Issue number1
DOIs
Publication statusPublished - Dec 27 1995

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Sodium-Hydrogen Antiporter
Primary Cell Culture
Prolactin
Thyrotropin-Releasing Hormone
Antiporters
Amiloride
Ouabain

Keywords

  • Amiloride analog
  • Na-Ca exchanger
  • Na-H antiporter
  • Prolactin

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Role of the Na+-Ca2+ and Na+-H+ antiporters in prolactin release from anterior pituitary cells in primary culture. / Di Renzo, Gianfranco; Amoroso, Salvatore; Bassi, Antonella; Fatatis, Alessandro; Cataldi, Mauro; Colao, Anna Maria; Lombardi, Gaetano; Annunziato, Lucio.

In: European Journal of Pharmacology, Vol. 294, No. 1, 27.12.1995, p. 11-15.

Research output: Contribution to journalArticle

Di Renzo, Gianfranco ; Amoroso, Salvatore ; Bassi, Antonella ; Fatatis, Alessandro ; Cataldi, Mauro ; Colao, Anna Maria ; Lombardi, Gaetano ; Annunziato, Lucio. / Role of the Na+-Ca2+ and Na+-H+ antiporters in prolactin release from anterior pituitary cells in primary culture. In: European Journal of Pharmacology. 1995 ; Vol. 294, No. 1. pp. 11-15.
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AB - 2′,4′-dimethylbenzamilamiloride (DMB), a somewhat selective inhibitor of the Na+-Ca2+ exchanger, in concentrations of 10, 30 and 100 μM did not produce any significant effect on baseline prolactin release from anterior pituitary cells in primary culture. When prolactin secretion was stimulated by the inhibitor of the Na+-K+-ATPase, ouabain, that activates the Na+-Ca2+ exchanger as a Ca2+-influx pathway, DMB was able to produce inhibition of prolactin secretion. 5-(N,N-hexamethylene) amiloride (HMA), another amiloride analog which specifically inhibits the Na+-H+ antiporter and has no inhibitory activity on the Na+-Ca2+ exchanger, at the concentrations of 0.1, 1 and 10 μM, did not affect basal prolactin release whereas it significantly reduced prolactin release stimulated by thyrotropin releasing hormone (TRH) (1 μM). These results suggest that the Na+-Ca2+ antiporter is involved in the process of prolactin release elicited by the inhibition of the Na+-K+-ATPase whereas the Na +-H+ antiporter is involved in the prolactin secretion elicited by TRH.

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