Role of the renal nerves in the control of renin synthesis during different sodium intakes in the rat

Raffaello Golin, Federico Pieruzzi, Carlotta Munforti, Giuseppe Busca, Anna Di Blasio, Alberto Zanchetti

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: The aim of this study was to evaluate the role of the renal nerves in the regulation of renin synthesis in normotensive rats at different sodium balance. Methods: Forty-eight male Sprague-Dawley rats were divided in six experimental groups, combining three diets at different NaCl content (normal 0.4%, low 0.04% or high 4.0%), and the surgical, bilateral renal denervation or the sham procedure. After 7 days of dietary treatment, all rats were sacrificed and plasma renin activity (PRA) was measured. Renin messenger RNA (mRNA) levels in the renal cortex were determined by semiquantitative polymerase chain reaction. Results: PRA was higher in animals fed the low sodium diet compared with those at standard diet, while it was lower in animals fed the high sodium diet. Renal denervation decreased PRA in normal and low sodium groups, while it did not alter the PRA values in the high sodium group. Renin gene expression significantly increased in rats fed with the low sodium diet compared with the standard diet group, and significantly decreased in rats fed the high sodium diet. Renal denervation significantly reduced renin mRNA levels in rats receiving the low sodium diet, but did not produce any significant change in normal or high-sodium groups. Conclusion: The activation of renin gene expression during sodium depletion in rats is dependent on the presence of the renal nerves, while the suppression of renin gene expression during a sodium load seems to be due to the macula densa mechanism alone.

Original languageEnglish
Pages (from-to)1271-1277
Number of pages7
JournalJournal of Hypertension
Volume19
Issue number7
DOIs
Publication statusPublished - 2001

Keywords

  • Plasma renin activity
  • Rats
  • Renal nerves
  • Renin synthesis
  • Sodium

ASJC Scopus subject areas

  • Endocrinology
  • Internal Medicine

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