Abstract
Restraint stress induces permeability changes in the small intestine, but little is known about the role of tumor necrosis factor (TNF)-α in the defects of the TJ function. In the present study, we used tumor necrosis factor-R1 knockout mice (TNF-α-R1KO) to understand the roles of TNF-α on ileum altered permeability function in models of immobilization stress. The genetic TNF-α inhibition significantly reduced the degree of 1) TNF-α production in ileum tissues; 2) the alteration of zonula occludens-1 (ZO-1), claudin-2, claudin-4, claudin-5, and β-catenin (immunohistochemistry); and 3) apoptosis (TUNEL staining, Bax, Bcl-2 expression). Taken together, our results demonstrate that inhibition of TNF-α reduces the tight junction permeability in the ileum tissues associated with immobilization stress, suggesting a possible role of TNF-α on ileum barrier dysfunction.
Original language | English |
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Journal | American Journal of Physiology - Gastrointestinal and Liver Physiology |
Volume | 294 |
Issue number | 5 |
DOIs | |
Publication status | Published - May 2008 |
Keywords
- β-catenin
- Apoptosis
- Claudin-2
- Tight junction
- TNF-α-deficient mice
- Zonula occludens-1
ASJC Scopus subject areas
- Physiology
- Gastroenterology
- Hepatology
- Physiology (medical)