Abstract
Restraint stress induces permeability changes in the small intestine, but little is known about the role of tumor necrosis factor (TNF)-α in the defects of the TJ function. In the present study, we used tumor necrosis factor-R1 knockout mice (TNF-α-R1KO) to understand the roles of TNF-α on ileum altered permeability function in models of immobilization stress. The genetic TNF-α inhibition significantly reduced the degree of 1) TNF-α production in ileum tissues; 2) the alteration of zonula occludens-1 (ZO-1), claudin-2, claudin-4, claudin-5, and β-catenin (immunohistochemistry); and 3) apoptosis (TUNEL staining, Bax, Bcl-2 expression). Taken together, our results demonstrate that inhibition of TNF-α reduces the tight junction permeability in the ileum tissues associated with immobilization stress, suggesting a possible role of TNF-α on ileum barrier dysfunction.
| Original language | English |
|---|---|
| Journal | American Journal of Physiology - Gastrointestinal and Liver Physiology |
| Volume | 294 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 2008 |
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Keywords
- β-catenin
- Apoptosis
- Claudin-2
- Tight junction
- TNF-α-deficient mice
- Zonula occludens-1
ASJC Scopus subject areas
- Physiology
- Gastroenterology
- Hepatology
- Physiology (medical)
Cite this
Role of TNF-α in ileum tight junction alteration in mouse model of restraint stress. / Mazzon, Emanuela; Cuzzocrea, Salvatore.
In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 294, No. 5, 05.2008.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Role of TNF-α in ileum tight junction alteration in mouse model of restraint stress
AU - Mazzon, Emanuela
AU - Cuzzocrea, Salvatore
PY - 2008/5
Y1 - 2008/5
N2 - Restraint stress induces permeability changes in the small intestine, but little is known about the role of tumor necrosis factor (TNF)-α in the defects of the TJ function. In the present study, we used tumor necrosis factor-R1 knockout mice (TNF-α-R1KO) to understand the roles of TNF-α on ileum altered permeability function in models of immobilization stress. The genetic TNF-α inhibition significantly reduced the degree of 1) TNF-α production in ileum tissues; 2) the alteration of zonula occludens-1 (ZO-1), claudin-2, claudin-4, claudin-5, and β-catenin (immunohistochemistry); and 3) apoptosis (TUNEL staining, Bax, Bcl-2 expression). Taken together, our results demonstrate that inhibition of TNF-α reduces the tight junction permeability in the ileum tissues associated with immobilization stress, suggesting a possible role of TNF-α on ileum barrier dysfunction.
AB - Restraint stress induces permeability changes in the small intestine, but little is known about the role of tumor necrosis factor (TNF)-α in the defects of the TJ function. In the present study, we used tumor necrosis factor-R1 knockout mice (TNF-α-R1KO) to understand the roles of TNF-α on ileum altered permeability function in models of immobilization stress. The genetic TNF-α inhibition significantly reduced the degree of 1) TNF-α production in ileum tissues; 2) the alteration of zonula occludens-1 (ZO-1), claudin-2, claudin-4, claudin-5, and β-catenin (immunohistochemistry); and 3) apoptosis (TUNEL staining, Bax, Bcl-2 expression). Taken together, our results demonstrate that inhibition of TNF-α reduces the tight junction permeability in the ileum tissues associated with immobilization stress, suggesting a possible role of TNF-α on ileum barrier dysfunction.
KW - β-catenin
KW - Apoptosis
KW - Claudin-2
KW - Tight junction
KW - TNF-α-deficient mice
KW - Zonula occludens-1
UR - http://www.scopus.com/inward/record.url?scp=45849127579&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=45849127579&partnerID=8YFLogxK
U2 - 10.1152/ajpgi.00014.2008
DO - 10.1152/ajpgi.00014.2008
M3 - Article
C2 - 18308862
AN - SCOPUS:45849127579
VL - 294
JO - American Journal of Physiology
JF - American Journal of Physiology
SN - 0363-6119
IS - 5
ER -