Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner, in neurite growth

Andrea Burgo, Emmanuel Sotirakis, Marie Christine Simmler, Agathe Verraes, Christophe Chamot, Jeremy C. Simpson, Letizia Lanzetti, Véronique Proux-Gillardeaux, Thierry Galli

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The vesicular soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) tetanus neurotoxin-insensitive vesicle-associated membrane protein (TI-VAMP/VAMP7) was previously shown to mediate an exocytic pathway involved in neurite growth, but its regulation is still largely unknown. Here we show that TI-VAMP interacts with the Vps9 domain and ankyrin-repeat-containing protein (Varp), a guanine nucleotide exchange factor (GEF) of the small GTPase Rab21, through a specific domain herein called the interacting domain (ID). Varp, TI-VAMP and Rab21 co-localize in the perinuclear region of differentiating hippocampal neurons and transiently in transport vesicles in the shaft of neurites. Silencing the expression of Varp by RNA interference or expressing ID or a form of Varp deprived of its Vps9 domain impairs neurite growth. Furthermore, the mutant form of Rab21, defective in GTP hydrolysis, enhances neurite growth. We conclude that Varp is a positive regulator of neurite growth through both its GEF activity and its interaction with TI-VAMP.

Original languageEnglish
Pages (from-to)1117-1124
Number of pages8
JournalEMBO Reports
Volume10
Issue number10
DOIs
Publication statusPublished - 2009

Fingerprint

Ankyrin Repeat
Neurites
Guanine Nucleotide Exchange Factors
Growth
Proteins
R-SNARE Proteins
SNARE Proteins
Transport Vesicles
Monomeric GTP-Binding Proteins
RNA Interference
Guanosine Triphosphate
Neurons
Hydrolysis
RNA

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Burgo, A., Sotirakis, E., Simmler, M. C., Verraes, A., Chamot, C., Simpson, J. C., ... Galli, T. (2009). Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner, in neurite growth. EMBO Reports, 10(10), 1117-1124. https://doi.org/10.1038/embor.2009.186

Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner, in neurite growth. / Burgo, Andrea; Sotirakis, Emmanuel; Simmler, Marie Christine; Verraes, Agathe; Chamot, Christophe; Simpson, Jeremy C.; Lanzetti, Letizia; Proux-Gillardeaux, Véronique; Galli, Thierry.

In: EMBO Reports, Vol. 10, No. 10, 2009, p. 1117-1124.

Research output: Contribution to journalArticle

Burgo, A, Sotirakis, E, Simmler, MC, Verraes, A, Chamot, C, Simpson, JC, Lanzetti, L, Proux-Gillardeaux, V & Galli, T 2009, 'Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner, in neurite growth', EMBO Reports, vol. 10, no. 10, pp. 1117-1124. https://doi.org/10.1038/embor.2009.186
Burgo A, Sotirakis E, Simmler MC, Verraes A, Chamot C, Simpson JC et al. Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner, in neurite growth. EMBO Reports. 2009;10(10):1117-1124. https://doi.org/10.1038/embor.2009.186
Burgo, Andrea ; Sotirakis, Emmanuel ; Simmler, Marie Christine ; Verraes, Agathe ; Chamot, Christophe ; Simpson, Jeremy C. ; Lanzetti, Letizia ; Proux-Gillardeaux, Véronique ; Galli, Thierry. / Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner, in neurite growth. In: EMBO Reports. 2009 ; Vol. 10, No. 10. pp. 1117-1124.
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