Role of vasostatin-1 C-terminal region in fibroblast cell adhesion

Eleonora Dondossola, Anna Gasparri, Angela Bachi, Renato Longhi, Marie Hélène Metz-Boutigue, Bruno Tota, Karen B. Helle, Flavio Curnis, Angelo Corti

Research output: Contribution to journalArticle

Abstract

Fibroblast adhesion can be modulated by proteins released by neuroendocrine cells and neurons, such as chromogranin A (CgA) and its N-terminal fragment vasostatin-1 (VS-1, CgA1-78). We have investigated the mechanisms of the interaction of VS-1 with fibroblasts and of its pro-adhesive activity and have found that the proadhesive activity of VS-1 relies on its interaction with the fibroblast membrane via a phospholipid-binding amphipathic α-helix located within residues 47-66, as well as on the interaction of the adjacent C-terminal region 67-78, which is structurally similar to ezrin-radixin-moesin- binding phosphoprotein 50 (a membrane-cytoskeleton adapter protein), with other cellular components critical for the regulation of cell cytoskeleton.

Original languageEnglish
Pages (from-to)2107-2118
Number of pages12
JournalCellular and Molecular Life Sciences
Volume67
Issue number12
DOIs
Publication statusPublished - Jun 2010

Keywords

  • Cell adhesion
  • Chromogranin A
  • Cytoskeleton
  • Ezrin
  • Ezrin-radixin-moesin-binding phosphoprotein 50
  • Vasostatin-I

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Molecular Medicine
  • Pharmacology
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Role of vasostatin-1 C-terminal region in fibroblast cell adhesion'. Together they form a unique fingerprint.

  • Cite this

    Dondossola, E., Gasparri, A., Bachi, A., Longhi, R., Metz-Boutigue, M. H., Tota, B., Helle, K. B., Curnis, F., & Corti, A. (2010). Role of vasostatin-1 C-terminal region in fibroblast cell adhesion. Cellular and Molecular Life Sciences, 67(12), 2107-2118. https://doi.org/10.1007/s00018-010-0319-5