Role of zinc-coordination and of the glutathione redox couple in the redox susceptibility of human transcription factor Sp1

L. Knoepfel, C. Steinkuhler, M. T. Carri, G. Rotilio

Research output: Contribution to journalArticle

Abstract

We show that thiol-groups confer redox-susceptibility to the zinc-finger transcription factor Sp1 and that this redox-susceptibility is prevented by DNA-binding and depends on zinc-coordination of the protein. Apo-Sp1 contained in metal depleted nuclear extracts of human K562 cells exhibited a markedly increased susceptibility towards oxidizing and alkylating agents, as compared to holo-Sp1. Moreover, DNA binding of apo-Sp1, but not of the holo-protein, was dramatically decreased in the presence of GSH/GSSG ratios within the physiological range. We compared these results with the redox behaviour of two other transcription factors, OTF-1 and NF1, which was found to be different in several aspects from that of Sp1.

Original languageEnglish
Pages (from-to)871-877
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume201
Issue number2
DOIs
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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