Roles for p53 and p73 during oligodendrocyte development

Nathalie Billon, Alessandro Terrinoni, Christine Jolicoeur, Afshan McCarthy, William D. Richardson, Gerry Melino, Martin Raff

Research output: Contribution to journalArticle

Abstract

Oligodendrocytes make myelin in the vertebrate central nervous system (CNS). They develop from oligodendrocyte precursor cells (OPCs), most of which divide a limited number of times before they stop and differentiate. OPCs can be purified from the developing rat optic nerve and stimulated to proliferate in serum-free culture by PDGF. They can be induced to differentiate in vitro by either thyroid hormone (TH) or PDGF withdrawal. It was shown previously that a dominant-negative form of p53 could inhibit OPC differentiation induced by TH but not by PDGF withdrawal, suggesting that the p53 family of proteins might play a part in TH-induced differentiation. As the dominant-negative p53 used inhibited all three known p53 family members - p53, p63 and p73 - it was uncertain which family members are important for this process. Here, we provide evidence that both p53 and p73, but not p63, are involved in TH-induced OPC differentiation and that p73 also plays a crucial part in PDGF-withdrawal-induced differentiation. This is the first evidence for a role of p73 in the differentiation of a normal mammalian cell.

Original languageEnglish
Pages (from-to)1211-1220
Number of pages10
JournalDevelopment
Volume131
Issue number6
DOIs
Publication statusPublished - Mar 2004

Keywords

  • Differentiation
  • Oligodendrocyte
  • p53
  • p63
  • p73
  • Rat

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

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    Billon, N., Terrinoni, A., Jolicoeur, C., McCarthy, A., Richardson, W. D., Melino, G., & Raff, M. (2004). Roles for p53 and p73 during oligodendrocyte development. Development, 131(6), 1211-1220. https://doi.org/10.1242/dev.01035