Roles of tumor necrosis factor p55 and p75 receptors in TNF-α-induced vascular permeability

Elisabetta Ferrero, Maria Raffaella Zocchi, Elena Magni, Maria Carla Panzeri, Flavio Curnis, Claudio Rugarli, Maria Elena Ferrero, Angelo Corti

Research output: Contribution to journalArticle

Abstract

We have investigated the role of p55 and p75 tumor necrosis factor receptors 1 and 2 (TNFR1 and TNFR2, respectively) in TNF-induced alteration of endothelial permeability in vitro and in vivo. Stimulation of TNFR1 with an agonist antibody or a receptor-selective TNF mutein increased the flux of 125I-albumin through endothelial cell monolayers. An antagonist anti-TNFR1 antibody, but not antagonist anti-TNFR2 antibodies, blocked the activity of TNF in vitro. Stimulation of TNFR1, but not TNFR2, induced cytoskeletal reorganization associated with increased permeability. SB-203580, a p38 mitogen-activated protein kinase inhibitor, blocked TNFR1-induced cytoskeletal reorganization and permeability. A selective mouse TNFR1 agonist and human TNF, which binds to murine TNFR1, increased the leakage of trypan blue-albumin from liver vessels in mice. These results indicate that stimulation of TNFR1 is necessary and sufficient to increase endothelial permeability in vitro and in vivo. However, an antagonist anti-murine TNFR2 antibody partially inhibited the effect of murine TNF on liver vessels, suggesting that TNFR2 also plays a role in the regulation of TNF-induced vascular permeability in vivo.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume281
Issue number4 50-4
Publication statusPublished - 2001

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Keywords

  • Cytoskeleton
  • Endothelium
  • Receptor

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

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