Rolly protein (ROLP)-Epb4.1/3: A potential protein-protein interaction relevant for the maintenance of cell adhesion

Manuele Castelnuovo, Massimiliano Monticone, Sara Massone, Irene Vassallo, Federico Tortelli, Ranieri Cancedda, Aldo Pagano

Research output: Contribution to journalArticlepeer-review

Abstract

We recently described Rolly Protein (ROLP), a small protein synthesized by substrate-adherent cells in a broad range of tissues. In a first set of experiments performed taking advantage of bone forming tibial cartilage as an experimental model we showed that ROLP transcription is associated to cells in an active proliferation state, whereas its downregulation is observed when cell proliferation decreases. Taking advantage of siRNA technology we also documented the expression modulation of some apoptosis-related genes in ROLP-silenced cells. In this work we search for the possible molecular interactors of ROLP by using both the antibody array approach as well as the co-immunoprecipitation approach. Results suggest the occurrence of an interaction of ROLP with Erythrocyte membrane Protein Band 4.1/3 (Epb4.1/3), an oncosuppressor downregulated in tumor development and in metastatic tissues; in addition we report experimental results that keep in line also with a potential interaction of ROLP with other PDZ-containing proteins. We also present experimental evidences supporting a role played by ROLP in cell adhesion thus supporting the existence of a biologically relevant link between ROLP and Epb4.1/3. We here suggest that ROLP might exert its biological role cooperating with Epb4.1/3, a protein that is involved in biological pathways that are often inhibited in tumor metastasis. Given the role of Epb4.1/3 in contrasting cancerogenesis we think that its cooperation with ROLP might be relevant in cancer studies and deserves further investigation.

Original languageEnglish
Pages (from-to)2054-2065
Number of pages12
JournalInternational Journal of Molecular Sciences
Volume10
Issue number5
DOIs
Publication statusPublished - May 2009

Keywords

  • Cell adhesion
  • Cell proliferation
  • Epb4.1/3
  • PSD-95
  • ROLP

ASJC Scopus subject areas

  • Computer Science Applications
  • Molecular Biology
  • Catalysis
  • Inorganic Chemistry
  • Spectroscopy
  • Organic Chemistry
  • Physical and Theoretical Chemistry

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