ROS1 rearrangements are uncommon in biliary tract cancers

Francesca Mazzoni, Paolo Petreni, Enrico Vasile, Michele Panebianco, Andrea Casadei‑Gardini, Francesca Negri, Alice Lunghi, Serena Pillozzi, Caterina Vivaldi, Erika Gervasi, Giovanni Luca Frassineti, Luca Messerini, Genny Jocollé, Alessandra Bisagni, Lorenzo Antonuzzo, Giulio Rossi

Research output: Contribution to journalArticlepeer-review


Biliary tract cancers (BTCs) are a pool of diseases with poor prognosis and there is no orphan drug available. Currently, no molecular targets have been tested as druggable oncogenic drivers. C‑ros oncogene 1 (ROS1) rearrangements have been previously described in various tumors, including BTCs; however, data regarding their incidence and biological significance are controversial. Therefore, a retrospective multi‑ center study was performed to assess the incidence of ROS1 rearrangements in BTCs by means of immunohistochemistry and fluorescence in situ hybridization (FISH). The present study failed to demonstrate ROS1 expression in a multicenter series of 150 cases with BTCs and revealed that D4D6 was the most specific clone compared with other ROS1 primary antibodies, namely PA1‑30318 and EPMGHR2. Notably, nega‑ tive results obtained with D4D6 completely matched to data sorted out by FISH analysis, thus confirming a lack of ROS1 gene rearrangements in BTCs and false positive results when PA1‑30318 and EPMGHR2 clones were used. These results suggest that ROS1 rearrangements may not be targets for molecular therapy of BTCs with specific inhibitors.

Original languageEnglish
Article number316
JournalOncology Letters
Issue number6
Publication statusPublished - Dec 1 2020


  • Biliary tract cancers
  • Biomarker
  • Fluorescence in situ hybridization
  • Immuno‑ histochemistry
  • Molecular target
  • ROS1 rearrangements

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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