Roughness of excimer laser ablated corneas with and without smoothing measured with atomic force microscopy

Marco Lombardo; Maria P. De Santo; Giuseppe Lombardo; Riccardo Barberi; Sebastiano Serrao

Roughness of excimer laser ablated corneas with and without smoothing measured with atomic force microscopy

Marco Lombardo, Maria P. De Santo, Giuseppe Lombardo, Riccardo Barberi, Sebastiano Serrao

Fondazione G.B. Bietti

Research output: Contribution to journal › Article › peer-review

Abstract

PURPOSE: To analyze the surface roughness of porcine corneas after excimer laser ablation with and without the smoothing procedure by means of atomic force microscopy. METHODS: Excimer laser photorefractive keratectomy (PRK) was performed on eight porcine corneas. Immediately following the procedure, smoothing was performed on four corneas using a viscous solution of 0.25% sodium hyaluronate. The corneas were examined in balanced salt solution after fixation in 2.5% glutaraldehyde solution using atomic force microscopy. Quantitative analysis of the ablated stromal surface topography was performed using the section analysis module of the atomic force microscopy software. Repeated measurements were made over small areas (≤50 μm²) near the center of each ablation, with a vertical resolution of

Original language	English
Pages (from-to)	469-475
Number of pages	7
Journal	Journal of Refractive Surgery
Volume	21
Issue number	5
Publication status	Published - Sep 2005

ASJC Scopus subject areas

Ophthalmology

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T1 - Roughness of excimer laser ablated corneas with and without smoothing measured with atomic force microscopy

AU - Lombardo, Marco

AU - De Santo, Maria P.

AU - Lombardo, Giuseppe

AU - Barberi, Riccardo

AU - Serrao, Sebastiano

PY - 2005/9

Y1 - 2005/9

N2 - PURPOSE: To analyze the surface roughness of porcine corneas after excimer laser ablation with and without the smoothing procedure by means of atomic force microscopy. METHODS: Excimer laser photorefractive keratectomy (PRK) was performed on eight porcine corneas. Immediately following the procedure, smoothing was performed on four corneas using a viscous solution of 0.25% sodium hyaluronate. The corneas were examined in balanced salt solution after fixation in 2.5% glutaraldehyde solution using atomic force microscopy. Quantitative analysis of the ablated stromal surface topography was performed using the section analysis module of the atomic force microscopy software. Repeated measurements were made over small areas (≤50 μm2) near the center of each ablation, with a vertical resolution of

AB - PURPOSE: To analyze the surface roughness of porcine corneas after excimer laser ablation with and without the smoothing procedure by means of atomic force microscopy. METHODS: Excimer laser photorefractive keratectomy (PRK) was performed on eight porcine corneas. Immediately following the procedure, smoothing was performed on four corneas using a viscous solution of 0.25% sodium hyaluronate. The corneas were examined in balanced salt solution after fixation in 2.5% glutaraldehyde solution using atomic force microscopy. Quantitative analysis of the ablated stromal surface topography was performed using the section analysis module of the atomic force microscopy software. Repeated measurements were made over small areas (≤50 μm2) near the center of each ablation, with a vertical resolution of

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Roughness of excimer laser ablated corneas with and without smoothing measured with atomic force microscopy

Abstract

ASJC Scopus subject areas

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