Rous sarcoma virus-transformed fibroblasts and cells of monocytic origin display a peculiar dot-like organization of cytoskeletal proteins involved in microfilament-membrane interactions

Pier Carlo Marchisio, Daniela Cirillo, Anna Teti, Alberta Zambonin-Zallone, Guido Tarone

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Abstract

By immunofluorescence and interference reflection microscopy (IRM) we found that F-actin and a group of cytoskeletal proteins involved in microfilament-membrane interaction, including vinculin, α-actinin, fimbrin and talin, are specifically organized in discrete dot-like structures corresponding to cell-substratum contact sites in both monocytes and monocyte-derived cells such as macrophages and osteoclasts. These proteins have a precise topological distribution; vinculin and talin form a doughnut-like ring, while actin, fimbrin and α-actinin are organized in dots matching the rings. An identical dot-like organization of F-actin and associated cytoskeletal proteins was also detected in malignant fibroblasts transformed by Rous Sarcoma virus (RSV) but not in the corresponding untransformed cells in culture. It is concluded that RSV transformation induces fibroblasts to express a cytoskeletal organization and a pattern of adhesion that are normally found in cells of monocytic origin. We propose that the occurrence of this cytoskeletal organization in RSV-transformed fibroblasts and in monocyte-derived cells may reflect a common ability to migrate across anatomical boundaries.

Original languageEnglish
Pages (from-to)202-214
Number of pages13
JournalExperimental Cell Research
Volume169
Issue number1
DOIs
Publication statusPublished - 1987

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Rous sarcoma virus
Cytoskeletal Proteins
Actin Cytoskeleton
Fibroblasts
Talin
Vinculin
Actinin
Actins
Monocytes
Membranes
Interference Microscopy
Osteoclasts
Fluorescent Antibody Technique
Cell Culture Techniques
Macrophages
Proteins
plastin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Rous sarcoma virus-transformed fibroblasts and cells of monocytic origin display a peculiar dot-like organization of cytoskeletal proteins involved in microfilament-membrane interactions. / Marchisio, Pier Carlo; Cirillo, Daniela; Teti, Anna; Zambonin-Zallone, Alberta; Tarone, Guido.

In: Experimental Cell Research, Vol. 169, No. 1, 1987, p. 202-214.

Research output: Contribution to journalArticle

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AU - Tarone, Guido

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