Rtf1-mediated eukaryotic site-specific replication termination

T. Eydmann, E. Sommariva, T. Inagawa, S. Mian, A. J S Klar, J. Z. Dalgaard

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Abstract

The molecular mechanisms mediating eukaryotic replication termination and pausing remain largely unknown. Here we present the molecular characterization of Rtf1 that mediates site-specific replication termination at the polar Schizosaccharomyces pombe barrier RTS1. We show that Rtf1 possesses two chimeric myb/SANT domains: one is able to interact with the repeated motifs encoded by the RTS1 element as well as the elements enhancer region, while the other shows only a weak DNA binding activity. In addition we show that the C-terminal tail of Rtf1 mediates self-interaction, and deletion of this tail has a dominant phenotype. Finally, we identify a point mutation in Rtf1 domain I that converts the RTS1 element into a replication barrier of the opposite polarity. Together our data establish that multiple protein DNA and protein-protein interactions between Rtf1 molecules and both the repeated motifs and the enhancer region of RTS1 are required for site-specific termination at the RTS1 element.

Original languageEnglish
Pages (from-to)27-39
Number of pages13
JournalGenetics
Volume180
Issue number1
DOIs
Publication statusPublished - Sep 2008

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ASJC Scopus subject areas

  • Genetics

Cite this

Eydmann, T., Sommariva, E., Inagawa, T., Mian, S., Klar, A. J. S., & Dalgaard, J. Z. (2008). Rtf1-mediated eukaryotic site-specific replication termination. Genetics, 180(1), 27-39. https://doi.org/10.1534/genetics.108.089243