TY - JOUR
T1 - S-100 protein and laminin
T2 - Immunocytochemical markers for human Schwann cells in vitro
AU - Scarpini, Elio
AU - Meola, Gianni
AU - Baron, Pierluigi
AU - Beretta, Sandro
AU - Velicogna, Mariaelena
AU - Scarlato, Guglielmo
PY - 1986
Y1 - 1986
N2 - Human fetal and adult Schwann cells, which had been maintained in culture for 5 weeks according to an explant-reexplantation technique, were labeled by immuno-peroxidase using antibodies directed against S-100 protein and laminin in order to find specific antigenic markers. Immunocytochemical analysis of the distribution of both proteins showed that they were expressed in long-term cultures. The localization of S-100 protein and laminin in long-term cultures indicated that the expression of these proteins by human Schwann cells was not axon-dependent and also occurred in absence of myelin synthesis.
AB - Human fetal and adult Schwann cells, which had been maintained in culture for 5 weeks according to an explant-reexplantation technique, were labeled by immuno-peroxidase using antibodies directed against S-100 protein and laminin in order to find specific antigenic markers. Immunocytochemical analysis of the distribution of both proteins showed that they were expressed in long-term cultures. The localization of S-100 protein and laminin in long-term cultures indicated that the expression of these proteins by human Schwann cells was not axon-dependent and also occurred in absence of myelin synthesis.
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U2 - 10.1016/0014-4886(86)90146-9
DO - 10.1016/0014-4886(86)90146-9
M3 - Article
C2 - 3732467
AN - SCOPUS:0022480441
VL - 93
SP - 77
EP - 83
JO - Experimental Neurology
JF - Experimental Neurology
SN - 0014-4886
IS - 1
ER -