S-adenosil-L-methionine is able to reverse the immunosuppressive effects of chenodeoxycholic acid in vitro

Gilberto Filaci, Nicoletta Pelli, Tommaso Sacco, Paola Contini, Lorella Lanza, Antonino Picciotto, Marco Scudeletti, Francesco Puppo, Graziella Castiglioni, Francesco Indiveri

Research output: Contribution to journalArticlepeer-review

Abstract

The study was conceived to evaluate if S-adenosil-L-methionine, a substance commonly used in the treatment of cholestasis in patients with cirrhosis and chronic hepatitis, exerts any immunological effect and if it is able to counterbalance bile acid-mediated immunosuppression. Proliferation and interleukin 2 and interferon-gamma secretion of human lymphocytes, collected from healthy subjects and exposed to mitogenic stimuli (phytohemagglutinin, pokeweed and anti-CD3 monoclonal antibodies), were analysed in the basal condition or after exposure to S-adenosil-L-methionine and/or chenodeoxycholic acid. Chenodeoxycholic acid inhibited phytohemagglutinin-induced lymphocyte proliferation and interferon-gamma secretion, and phytohemagglutinin and pokeweed-mediated interleukin 2 secretion. S-adenosil-L-methionine did not affect lymphocyte proliferation while it reduced interleukin 2 secretion upon phytohemagglutinin and pokeweed stimulation and interferon-gamma secretion upon all stimuli tested. Moreover, S-adenosil-L-methionine counteracted chenodeoxycholic acid-mediated inhibition of lymphocyte proliferation and interleukin 2 secretion. The results of our study confirm the immunosuppressive role of chenodeoxycholic acid on both secretive and proliferative lymphocyte functions and provide evidence of immunomodulatory activities of S-adenosil-L-methionine and its capacity to antagonize chenodeoxycholic acid-mediated inhibition of lymphocyte proliferation and interleukin 2 secretion.

Original languageEnglish
Pages (from-to)157-165
Number of pages9
JournalInternational Journal of Immunopharmacology
Volume19
Issue number3
DOIs
Publication statusPublished - Mar 1997

Keywords

  • Chenodeoxycholic acid
  • Immunosuppression
  • S-adenosil-L-methionine

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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